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Data from: Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms
负责人:
关键词:
DNA Barcoding;metagenomics;Bacillariophyta;diatoms;Algae;Community Ecology
DOI:
doi:10.5061/dryad.370nq
摘要:
The recent emergence of barcoding approaches coupled to those of Next Generation Sequencing (NGS) have raised new perspectives for studying
-enriched shotgun pyrosequencing.
负责人:
关键词:
high throughput genotyping;Next-generation sequencing;short sequence repeats;Alnus incana;Alnus cordata;common alder;Alnus glutinosa;454 GS-FLX Titanium
DOI:
doi:10.5061/dryad.gg0jm
摘要:
Recent developments in sequencing technologies and bioinformatics analyses provide an unprecedented opportunity for cost and time effect
Data from: Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes
负责人:
关键词:
Polymorphic membrane proteins Chlamydial cytotoxins Ribosomal proteins
DOI:
doi:10.5061/dryad.np597
摘要:
PV3056/3) were determined using Illumina/Solexa and Roche 454 genome sequencing. Results: Gene order and synteny was almost identical between C. pecorum
Data from: The effects of contig length and depth on the estimation of SNP frequencies, and the relative abundance of SNPs in protein-coding
负责人:
关键词:
454 sequencing;Contig depth;SNP frequency;model selection;genomics;transcriptome;Ambystoma tigrinum;Contig length
DOI:
doi:10.5061/dryad.5pd17
摘要:
BACKGROUND: Next-generation sequencing methods have contributed to rapid progress in the fields of genomics and population genetics. Using this high
Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife
负责人:
关键词:
NGS RNA-Seq 16S metagenomics metabarcoding 454-pyrosequencing MiSeq bacteria rodent-borne zoonoses coinfections pathobiome
DOI:
doi:10.5061/dryad.50125
摘要:
s not been assessed. Here, we compared two NGS approaches, RNA-Sequencing (RNA-Seq) and 16S-metagenomics, assessing their ability to survey neglected zoonotic bacteria
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
负责人:
关键词:
DNA metabarcoding;Chimeric sequences;PCR bias;UCHIME;microbial ecology
DOI:
doi:10.5061/dryad.n4cv3
摘要:
and 454 sequencing of the ITS2 region from fungal mock communities of different complexities. We evaluated the chimera detecting ability of two common chimera-checking
Data from: High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries
负责人:
Malausa, Thibaut
关键词:
Population Genetics - Empirical Genomics/Proteomics Bioinfomatics/Phyloinfomatics Fungi Insects genetic markers
DOI:
doi:10.5061/dryad.8297
摘要:
e a high-throughput method for isolating microsatellite markers based on coupling multiplex microsatellite enrichment and Next-Generation Sequencing on 454
Data from: Data concatenation, Bayesian concordance and coalescent-based analyses of the species tree for the rapid radiation of Triturus newts
负责人:
关键词:
454 sequencing;crested newt;Gene tree;transcriptome;multi-species coalescent;Triturus;marbled newt
DOI:
doi:10.5061/dryad.mm81p
摘要:
-coding genes, obtained with 454 sequencing. Our dataset includes twenty Triturus newts and represents all nine species. Bayesian analysis of population structure
Data from: Molecular study of bacterial diversity within the trophosome of the vestimentiferan tubeworm Ridgeia piscesae
负责人:
关键词:
vestimentiferans;Ridgeia piscesae;454-pyrosequencing;Hydrothermal vents;symbiosis;catalyzed reporter deposition-fluorescence in situ hybridization;Sanger sequencing
DOI:
doi:10.5061/dryad.4gk80
摘要:
in R. piscesae using two methodological approaches not yet applied to this symbiosis. We carried out 454-pyrosequencing on trophosome samples from 46 individual
Data from: Analyzing reticulate relationships using CpDNA and pyrosequenced ITS1 as exemplified by Veronica subgen. Pseudolysimachium (Plantaginaceae)
负责人:
关键词:
454 sequencing;Veronica subgen. Pseudolysimachium;Veronica;ITS;hybridization;Pseudolysimachium;cpDNA;steppe
DOI:
doi:10.5061/dryad.bc111
摘要:
). Our second approach, pyrosequencing of ITS1, generated up to nine different unique sequences per individual and phylogenetic analyses of the datase

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