筛选
科学数据
统计数据
共检索到40条 ,权限内显示50条;
Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife
- 负责人：
- 关键词：
- MiSeq NGS RNA-Seq 16S metagenomics metabarcoding 454-pyrosequencing bacteria rodent-borne zoonoses coinfections pathobiome
- DOI：
- doi:10.5061/dryad.50125
- 摘要：
- . DNA extracts were analyzed via a 16S-metagenomics approach using two sequencers: the 454 GS-FLX and the MiSeq. The V4 region of the gene coding for 16S rRNA
Data from: CHIIMP: an automated high-throughput microsatellite genotyping approach reveals greater allelic diversity in wild chimpanzees
- 负责人：
- DOI：
- doi:10.5061/dryad.59j3974
- 摘要：
- r, this technique is labor intensive, difficult to compare across platforms, and notoriously imprecise. Here we developed a MiSeq-based approach and tested
Data from: Species tree estimation of North American chorus frogs (Hylidae: Pseudacris) with parallel tagged amplicon sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.23rc0
- 摘要：
- for 44 individuals on 1/3 of an Illumina MiSeq run, obtaining 96.5% of the targeted amplicons at less than 20% of the cost of traditional Sanger se
Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar
- 负责人：
- DOI：
- doi:10.5061/dryad.591q3
- 摘要：
- and Illumina MiSeq, we generated nuclear, subgenomic libraries for 96 species simultaneously and sequenced them for a total cost of ca. $6000 USD. Approximately half
Data from: Bacterial characterization of Beijing drinking water by flow cytometry and MiSeq sequencing of the 16S rRNA gene
- 负责人：
- DOI：
- doi:10.5061/dryad.55nb0
- 摘要：
- r, they provided a health risk for special crowd, such as the elderly or infants, patients with burns and immune-compromised people exposed by drinking
Data from: Cost-effective enrichment hybridization capture of chloroplast genomes at deep multiplexing levels for population genetics and phylogeography studies
- 负责人：
- 关键词：
- MiSeq DNA probes long range PCR next generation sequencing plastomes whole chloroplast sequencing
- DOI：
- doi:10.5061/dryad.t6b05
- 摘要：
- the effectiveness of this protocol to retrieve 95% of the sequence of the whole chloroplast on 95 multiplexed individuals in a single MiSeq ru
Data from: A from-benchtop-to-desktop workflow for validating HTS data and for taxonomic identification in diet metabarcoding studies
- 负责人：
- DOI：
- doi:10.5061/dryad.f40v5
- 摘要：
- was amplified by combining two minibarcoding primer sets to maximize the taxonomic coverage. Amplicons were sequenced by an Illumina MiSeq platform. We developed
Data from: Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq)
- 负责人：
- DOI：
- doi:10.5061/dryad.mf1ck
- 摘要：
- in quality from nearly intact to highly sheared. All samples were sequenced as a multiplexed pool on an Illumina MiSeq. Libraries created from low
Data from: Disease-free monoculture farming by fungus-growing termites
- 负责人：
- DOI：
- doi:10.5061/dryad.t6t12
- 摘要：
- termite species (two genera). Internal Transcribed Spacer (ITS) gene analyses using 454 pyrosequencing and Illumina MiSeq showed that non-Termitomyces fungi
Data from: Isolation, characterization, and cross-amplification of 20 microsatellite markers for the rare Conospermum undulatum (Proteaceae)
- 负责人：
- DOI：
- doi:10.5061/dryad.f81k3q7
- 摘要：
- rvation purposes and to facilitate ecological studies. Methods and Results: Illumina MiSeq high-throughput sequencing was used to develop 20 nove
