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Data from: 18S rRNA V9 metabarcoding for diet characterization: a critical evaluation with two sympatric zooplanktivorous fish species
- 负责人:
- 关键词:
- DOI:
- doi:10.5061/dryad.2p1t8
- 摘要:
- The potential of the 18S rRNA V9 metabarcoding approach for diet assessment was explored using MiSeq paired-end (PE; 2 × 150 bp) technology
Data from: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical
- 负责人:
- DOI:
- doi:10.5061/dryad.54v2q
- 摘要:
- We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned
Data from: Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data improve genotype calling accuracy
- 负责人:
- DOI:
- doi:10.5061/dryad.34dt1
- 摘要:
- four-base tag to the first index read, which allows duplicate discrimination in aligned data. This approach was validated on the Illumina MiSeq platform, usi
Data from: A broadly applicable COI primer pair and an efficient single?tube amplicon library preparation protocol for metabarcoding
- 负责人:
- 关键词:
- DOI:
- doi:10.5061/dryad.5jq5t36
- 摘要:
- samples were sequenced on a MiSeq platform with no adverse effects compared to commercial Nextera indexing kits. From this run 75% of all included taxa
Data from: Benchmarking DNA metabarcoding for biodiversity-based monitoring and assessment
- 负责人:
- DOI:
- doi:10.5061/dryad.0sc0s
- 摘要:
- , traditionally and widely used for species identification in coastal and marine ecosystem communities, is tedious and entails limitations. Metabarcoding coupled
Data from: Microbe biogeography tracks water masses in a dynamic oceanic frontal system
- 负责人:
- DOI:
- doi:10.5061/dryad.qh767
- 摘要:
- quencing of the 16S rRNA gene on an Illumina MiSeq platform. Multivariate analyses showed that microbial communities were structured more strongly by depth than by latitude
Data from: Environmental filtering by pH and soil nutrients drives community assembly in fungi at fine spatial scales
- 负责人:
- DOI:
- doi:10.5061/dryad.q0rv9
- 摘要:
- Whether niche processes, like environmental filtering, or neutral processes, like dispersal limitation, are the primary forces driving
by Illumina sequencing
- 负责人:
- 关键词:
- Hololena adnexa
- DOI:
- doi:10.5061/dryad.311mm
- 摘要:
- ral hundred predators in a single MiSeq run, reducing the cost per specimen to only a few dollars and requiring a simple and minimal work flow
Data from: Spatial and successional dynamics of microbial biofilm communities in a grassland stream ecosystem
- 负责人:
- DOI:
- doi:10.5061/dryad.m2r17
- 摘要:
- il 64 days suggesting a balance of the use of autochthonous and allochthonous energy sources until late succession. Bacterial communities (MiSeq analyse
Data from: Fungal community reveals less dispersal limitation and potentially more connected network than that of bacteria in bamboo forest soils
- 负责人:
- 关键词:
- DOI:
- doi:10.5061/dryad.33th5
- 摘要:
- and fungal diversities were assessed by sequencing 16S rRNA and ITS gene amplicons, respectively, with an Illumina MiSeq. Based on structural equation modeling