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Data from: Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community
- 负责人：
- DOI：
- doi:10.5061/dryad.tn6vn
- 摘要：
- community-wide sequencing effort. Here we use Fluidigm’s micro-fluidic Access Arrays (IFC) followed by Illumina? MiSeq Nano sequencing on a culture-derived
Data from: Comparison and validation of Oomycetes metabarcoding primers for Phytophthora high throughput sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.68tk49g
- 摘要：
- . The three nested PCR strategies proved to be a reliable qualitative approach, identifying approximately 95% of the species after Illumina Miseq se
Data from: Comparing microbiota profiles in induced and spontaneous sputum samples in COPD patients
- 负责人：
- DOI：
- doi:10.5061/dryad.5gc82
- 摘要：
- -end sequencing. Illumina Miseq System was used for sequencing, and Quantitative Insights Into Microbial Ecology (QIIME) and Stata were used for bioinformatics
Data from: Analysis of microsatellite loci in tree of heaven (Ailanthus altissima (Mill.) Swingle) using SSR-GBS
- 负责人：
- 关键词：
- genotyping by sequencing;SNP;microsatellite;Amplicon Sequencing;Ailanthus altissima;Tree of heaven
- DOI：
- doi:10.5061/dryad.873b2j2
- 摘要：
- w microsatellite markers for the tree of heaven by applying an NGS-based method using the Illumina MiSeq platform. NGS technology was proved to be an effective method
Data from: 2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador
- 负责人：
- DOI：
- doi:10.5061/dryad.02bf1
- 摘要：
- collected from the central coast and southern Andean region of Ecuador, from June 2006 to July 2013. 2b-RAD sequencing data was performed on an Illumina MiSeq
Data from: Metabarcoding and mitochondrial metagenomics of endogean arthropods to unveil the mesofauna of the soil
- 负责人：
- DOI：
- doi:10.5061/dryad.5t5s1
- 摘要：
- of arthropod communities from these bulk DNA extractions using cox1 metabarcoding and shotgun metagenomic sequencing on the MiSeq Illumina platform. The FBF protocol
Data from: Family-assisted inference of the genetic architecture of MHC variation
- 负责人：
- DOI：
- doi:10.5061/dryad.rm611
- 摘要：
- ), including 590 offspring from 130 families using Illumina MiSeq technology. Coupled with a high per-individual sequencing coverage (~3000×), the study
Data from: Shifts of tundra bacterial and archaeal communities along a permafrost thaw gradient in Alaska
- 负责人：
- DOI：
- doi:10.5061/dryad.p1602
- 摘要：
- This study investigated soil bacterial and archaeal communities by Illumina MiSeq sequencing of 16S rRNA gene amplicons across a permafrost thaw gradient
Data from: The effects of read length, quality and quantity on microsatellite discovery and primer development: from Illumina to PacBio
- 负责人：
- DOI：
- doi:10.5061/dryad.r20t0
- 摘要：
- , while platforms such as Illumina MiSeq will provide cost-efficient solutions for multi-species microsatellite projects.
Data from: Isolation of microsatellite loci by next-generation sequencing of the critically endangered Northern Bald ibis, Geronticus eremita
- 负责人：
- DOI：
- doi:10.5061/dryad.h1167
- 摘要：
- microsatellite markers, specific for the Northern Bald Ibis, Geronticus eremita (LINNAEUS, 1785), have been isolated from next-generation sequencing (Illumina MiSeq
