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Data from: Lessons learned from microsatellite development for non-model organisms using 454 pyrosequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.22p7m
- 摘要：
- . Next Generation Sequencing techniques such as 454 pyrosequencing allow the rapid development of microsatellite markers in nonmodel organisms. 454 pyrosequencing
Data from: Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)
- 负责人：
- DOI：
- doi:10.5061/dryad.q0740kp8
- 摘要：
- . In particular, 454 pyrosequencing of nonmodel species has been used to characterize expressed sequence tags, xenobiotics, gene ontologies, and r
Data from: Comparison of 454 pyrosequencing methods for characterizing the major histocompatibility complex of nonmodel species and the advantages
- 负责人：
- DOI：
- doi:10.5061/dryad.s5b40
- 摘要：
- s of assessment often underestimate total variation. Here, we explored the suitability of 454 pyrosequencing for characterizing multilocus genes for use
Data from: Cross-species transferability of SSR loci developed from transcriptome sequencing in lodgepole pine
- 负责人：
- DOI：
- doi:10.5061/dryad.8dk6t78s
- 摘要：
- ulting from 454 pyrosequencing of Pinus contorta cDNA were identified. From these we developed PCR primers for approximately 4000 candidate SSRs. Her
Data from: Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens
- 负责人：
- DOI：
- doi:10.5061/dryad.p25vn
- 摘要：
- to DNA barcoding PCR primers. We employ 454 pyrosequencing to recover full-length DNA barcodes of 190 specimens using 12.5% capacity of a 454 sequencing ru
Data from: A next-generation sequencing approach to river biomonitoring using benthic diatoms
- 负责人：
- DOI：
- doi:10.5061/dryad.3gm8c
- 摘要：
- . Next Generation Sequencing (NGS) can be used to study communities of microorganisms, so we carried out a test of the reliability of 454 pyrosequencing for estimating diatom
Data from: Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms
- 负责人：
- DOI：
- doi:10.5061/dryad.370nq
- 摘要：
- was constituted directly from pooled cultures, whereas the others consisted of pooled PCR products. The NGS reads obtained by pyrosequencing (Roche 454) wer
Data from: Tissue storage and primer selection influence pyrosequencing-based inferences of diversity and community composition of endolichenic
- 负责人：
- DOI：
- doi:10.5061/dryad.8271f
- 摘要：
- d in 454 amplicon pyrosequencing might influence ecological inferences in studies of endophytic and endolichenic fungi. We first contrast 454 data se
-enriched shotgun pyrosequencing.
- 负责人：
- DOI：
- doi:10.5061/dryad.gg0jm
- 摘要：
- ive high quality microsatellite marker discovery in non-model organisms for which no genomic information is available. Here, we use shotgun pyrosequencing
Data from: Temporal dynamics of plant-soil feedback and root-associated fungal communities over 100 years of invasion by a non-native plant
- 负责人：
- DOI：
- doi:10.5061/dryad.vp530
- 摘要：
- ot fungal communities in the context of invasion time. 2. We used a plant-soil feedback study and 454 pyrosequencing to investigate pathogen accumulation over 100 yea
