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Data from: The effects of contig length and depth on the estimation of SNP frequencies, and the relative abundance of SNPs in protein-coding
- 负责人:
- DOI:
- doi:10.5061/dryad.5pd17
- 摘要:
- methods of SNP frequency estimation. RESULTS: We used 454 pyrosequencing to identify 2,980 putative SNPs in the eastern tiger salamander (Ambystoma tigrinum
Data from: Photoautotrophic symbiont and geography are major factors affecting highly structured and diverse bacterial communities in the lichen
- 负责人:
- DOI:
- doi:10.5061/dryad.t99b1
- 摘要:
- were examined using both Sanger sequencing of cloned fragments and barcoded pyrosequencing. Rhizobiales is typically the most abundant and taxonomically
Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife
- 负责人:
- 关键词:
- NGS RNA-Seq 16S metagenomics metabarcoding 454-pyrosequencing MiSeq bacteria rodent-borne zoonoses coinfections pathobiome
- DOI:
- doi:10.5061/dryad.50125
- 摘要:
- -MiSeq). In contrast, 21 of these pathogens went unnoticed when the 16S-metagenomics approach was processed via 454-pyrosequencing (16S-454). In addition
Data from: Assembly of root-associated bacteria communities: interactions between abiotic and biotic factors
- 负责人:
- DOI:
- doi:10.5061/dryad.7535k
- 摘要:
- ng 454 pyrosequencing, we examined RAB communities from two dominant alpine tundra plants, Geum rossii and Deschampsia cespitosa, under control, N addition
by pyrosequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.69t48k87
- 摘要:
- s. We do so with a hierarchical experimental design that captures variation in expression among genes, among tissues, and among individuals. Using 454 pyrosequencing, we pro
pyrosequencing
- 负责人:
- 关键词:
- metagenomics;DNA Barcoding;Mus musculus;Quaternary;bacteria;house mouse;Community Ecology;speciation
- DOI:
- doi:10.5061/dryad.018g4
- 摘要:
- and variation in wild populations is poorly understood. Using 454 pyrosequencing, we performed 16S rDNA GTM barcoding for 30 wild house mice (Mus musculus
Data from: Plant host and soil origin influence fungal and bacterial assemblages in the roots of woody plants
- 负责人:
- DOI:
- doi:10.5061/dryad.2b2dj
- 摘要:
- field sites. Fungal and bacterial community profiles in the root of each replicate were assessed through multiplex 454 amplicon sequencing of 4 loci
Data from: Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.b8d21
- 摘要:
- operational taxonomic units (OTUs), from c. 400.000 454 pyrosequencing reads, after rigorous bioinformatic filtering and clustering at 99.5%. Most OTUs represented unc
Data from: A metagenetic approach for revealing community structure of marine planktonic copepods
- 负责人:
- DOI:
- doi:10.5061/dryad.5p1j3
- 摘要:
- ach for revealing the community structure of marine planktonic copepods using 454 pyrosequencing of nuclear large subunit ribosomal DNA. We determined an appro
Data from: Isolation and characterization of microsatellite loci for the isopod crustacean Armadillidium vulgare and transferability
- 负责人:
- DOI:
- doi:10.5061/dryad.md545
- 摘要:
- duction strategies. To provide molecular tools for examining these questions, we isolated microsatellite loci through 454 pyrosequencing of a repeat-enriched