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Data from: The QRS (Quantification of Representative Sequences) pipeline for amplicon sequencing: case study on within-population ITS1
- 负责人：
- DOI：
- doi:10.5061/dryad.v7f7r
- 摘要：
- whether the estimated frequency of representative sequences, generated by 454 amplicon sequencing, differs significantly from that obtaine
Data from: Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens
- 负责人：
- DOI：
- doi:10.5061/dryad.p25vn
- 摘要：
- , inferior to next-generation sequencers, which are capable of producing millions of sequence reads simultaneously. Additionally, direct Sanger sequencing
Data from: Lessons learned from microsatellite development for non-model organisms using 454 pyrosequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.22p7m
- 摘要：
- . Next Generation Sequencing techniques such as 454 pyrosequencing allow the rapid development of microsatellite markers in nonmodel organisms. 454 pyrosequencin
Data from: SSR_pipeline: a bioinformatic infrastructure for identifying microsatellites from paired-end Illumina high-throughput DNA sequencing data
- 负责人：
- DOI：
- doi:10.5061/dryad.n65k2
- 摘要：
- -throughput Illumina DNA sequencing data. The program suite contains 3 analysis modules along with a fourth control module that can automate analyse
Data from: Species level phylogeny and polyploid relationships in Hordeum (Poaceae) inferred by next-generation sequencing and in-silico cloning
- 负责人：
- DOI：
- doi:10.5061/dryad.fn2nt
- 摘要：
- ed in a single run on a 454 platform. Reference sequences were obtained by cloning and Sanger sequencing of all loci for nine supplementary individuals. The 454
Data from: High-throughput microsatellite marker development in two sparid species and verification of their transferability in the family Sparidae
- 负责人：
- DOI：
- doi:10.5061/dryad.320kb06t
- 摘要：
- Recently, 454 sequencing has emerged as a popular method for isolating microsatellites owing to cost-effectiveness and time saving. In this study
Data from: Microsatellite markers from the Ion Torrent: a multi-species contrast to 454 shotgun sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.vv82q
- 摘要：
- pyro-sequencing (454). More recent platforms such as the PGM semiconductor sequencer (Ion Torrent) offer potential benefits such as dramatic reductions
Data from: Comparison of 454 pyrosequencing methods for characterizing the major histocompatibility complex of nonmodel species and the advantages
- 负责人：
- DOI：
- doi:10.5061/dryad.s5b40
- 摘要：
- is challenging in nonmodel vertebrates. The traditional method of extensive cloning and Sanger sequencing is costly and time-intensive and indirect method
Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.
- 负责人：
- Brelsford, Alan
- DOI：
- doi:10.5061/dryad.1hg75
- 摘要：
- High-fidelity “proofreading” polymerases are often used in library construction for next-generation sequencing projects, in an effort to minimize
Data from: Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies
- 负责人：
- DOI：
- doi:10.5061/dryad.6fm1p
- 摘要：
- During the past decade, DNA sequencing output has been mostly dominated by the second generation sequencing platforms which are characterized
