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Data from: Pitfalls during in silico prediction of primer specificity for eDNA surveillance
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.41ns1rnb7
- 摘要：
- of 12 primer pairs were further tested with SYBR Green-based or TaqMan-based singleplex quantitative PCR (qPCR). We disentangle the relative importa
Data from: Development and validation of rapid environmental DNA (eDNA) detection methods for bog turtle (Glyptemys muhlenbergii)
- 负责人：
- DOI：
- doi:10.5061/dryad.6n7675r
- 摘要：
- can be ineffective due to the turtle’s wetland habitat, small size, and burrowing nature. Molecular methods, such as qPCR, provide the ability to ove
Data from: Clearing muddied waters: capture of environmental DNA from turbid waters
- 负责人：
- DOI：
- doi:10.5061/dryad.835s6
- 摘要：
- to quantitative PCR and found that quantitative PCR was more sensitive in detecting lower concentrations of eDNA. We show significant differences in efficiencies amo
Data from: Effects of prey quality and predator body size on prey DNA detection success in a centipede predator
- 负责人：
- DOI：
- doi:10.5061/dryad.ss17b
- 摘要：
- cond hypothesis. However, sensitivity of diagnostic PCR differed between prey types and quantitative PCR revealed that concentration of targeted DNA varied significant
Data from: Increasing the accuracy and precision of relative telomere length estimates by RT qPCR
- 负责人：
- DOI：
- doi:10.5061/dryad.7356h
- 摘要：
- length analysis with in-gel hybridisation), could be improved. We find qPCR repeatability (intra- and inter-assay levels) to be at similar levels across thr
Data from: Environmental DNA analysis of river herring in Chesapeake Bay: a powerful tool for monitoring threatened keystone species
- 负责人：
- DOI：
- doi:10.5061/dryad.n60t8b3
- 摘要：
- presence and abundance data to traditional methods of quantification (ichthyoplankton sampling and adult observations). Overall, the qPCR assay showed ve
Data from: Factors influencing detection of eDNA from a stream-dwelling amphibian
- 负责人：
- DOI：
- doi:10.5061/dryad.mt60p
- 摘要：
- ng stream-dwelling salamanders and quantitative PCR (qPCR) analysis, we conducted three experiments to assess eDNA: (1) production rate, (2) persistence ti
Data from: Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data improve genotype calling accuracy
- 负责人：
- DOI：
- doi:10.5061/dryad.34dt1
- 摘要：
- with inflated confidence as a result of PCR duplicates. Quantification of library complexity by adaptor tagging does not significantly increase the difficulty
Data from: The differential impact of a native and a non-native ragwort species (Senecioneae) on the first and second trophic leve
- 负责人：
- DOI：
- doi:10.5061/dryad.t4313
- 摘要：
- ken into consideration. Quantitative PCR assays for the analyses of bacterial and fungal communities at a high taxonomic level were opti
Data from: MicroRNA stability in FFPE tissue samples: dependence on GC content
- 负责人：
- 关键词：
- human;MiRNA
- DOI：
- doi:10.5061/dryad.fj0f8
- 摘要：
- in FFPE cardiac tissues instead of miR-1, which was predominant before fixation. Subsequent quantitative PCR (qPCR) analyses revealed that miRNAs
