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Data from: Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation
- 负责人：
- DOI：
- doi:10.5061/dryad.1rn8pk0qz
- 摘要：
- and multi-trophic level interactions. Both conventional polymerase chain reaction (cPCR) and quantitative PCR (qPCR) have been employed to detect predation
Data from: A whole-transcriptome approach to evaluating reference genes for quantitative gene expression studies: a case study in Mimulus
- 负责人：
- DOI：
- doi:10.5061/dryad.84655
- 摘要：
- While quantitative PCR (qPCR) is widely recognized as being among the most accurate methods for quantifying gene expression, it is highly dependent
Data from: Pitfalls during in silico prediction of primer specificity for eDNA surveillance
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.41ns1rnb7
- 摘要：
- of 12 primer pairs were further tested with SYBR Green-based or TaqMan-based singleplex quantitative PCR (qPCR). We disentangle the relative importance
Data from: Increasing the accuracy and precision of relative telomere length estimates by RT qPCR
- 负责人：
- DOI：
- doi:10.5061/dryad.7356h
- 摘要：
- , the costs of reproduction and disease. qPCR is a high throughput and cost effective tool to measure relative TL (rTL) that can be applied to newly-collected and archived
Data from: 60 specific eDNA qPCR assays to detect invasive, threatened and exploited freshwater vertebrates and invertebrates in Eastern Canada
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.12jm63xtw
- 摘要：
- for which monitoring may be challenging. Species detection efforts using a combination of eDNA and qPCR have been highly successful and, as a result, thei
Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
- 负责人：
- DOI：
- doi:10.5061/dryad.28n8d6t
- 摘要：
- in the availability of antibodies for detecting proteins, quantitative PCR (qPCR) remains the preferred method for quantifying target gene knockdown after dsRNA treatment
Data from: Development and validation of rapid environmental DNA (eDNA) detection methods for bog turtle (Glyptemys muhlenbergii)
- 负责人：
- DOI：
- doi:10.5061/dryad.6n7675r
- 摘要：
- can be ineffective due to the turtle’s wetland habitat, small size, and burrowing nature. Molecular methods, such as qPCR, provide the ability to overcome
Data from: Fold or hold: experimental evolution in vitro
- 负责人：
- DOI：
- doi:10.5061/dryad.1nt11
- 摘要：
- quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures tha
Data from: Blood-stage parasitaemia and age determine Plasmodium falciparum and P. vivax gametocytaemia in Papua New Guinea
- 负责人：
- DOI：
- doi:10.5061/dryad.550p3
- 摘要：
- carriage, 2083 samples were collected in a cross-sectional survey in Papua New Guinea. Plasmodium species were detected by light microscopy and qPCR
Data from: Design- and model-based strategies for detecting and quantifying an amphibian pathogen in environmental samples
- 负责人：
- DOI：
- doi:10.5061/dryad.2j0h3
- 摘要：
- . We used a laboratory experiment to evaluate the influences of pathogen concentration, water type, and qPCR inhibitors on the detection and quantification
