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Data from: Neonatal activation of the xenobiotic-sensors PXR and CAR results in acute and persistent down-regulation of PPAR?-signaling in mouse liver
负责人:
关键词:
mouse;mouse;Drug-processing genes;Nuclear receptors;Nuclear receptors
DOI:
doi:10.5061/dryad.0k90t
摘要:
gene loci. RT-qPCR, western blotting, and enzyme activity assays in livers of wild-type, PXR-null, and CAR-null mice confirmed that the persistent
Data from: Environmental DNA (eDNA) detects the invasive rusty crayfish (Orconectes rusticus) at low abundances
负责人:
关键词:
Orconectes rusticus;quantitative PCR;exotic species;crayfish;early warning;detection probability;early detection;Freshwater;occupancy estimation;Lake;non-indigenous species
DOI:
doi:10.5061/dryad.3hp15
摘要:
conventional baited trapping as a measure of crayfish relative abundance with water samples for eDNA, which were analysed in the laboratory with a qPCR
Data from: A metatranscriptomic analysis of diseased social wasps (Vespula vulgaris) for pathogens, with an experimental infection of larvae
负责人:
关键词:
pathogens;2016;biological control;Vespula vulgaris;RNAseq;invasive species
DOI:
doi:10.5061/dryad.m98pt3k
摘要:
t we monitored, however, had a significantly higher prevalence in diseased than in healthy larvae. RT-qPCR analysis indicated that pathogen infections were signi
Data from: Seasonal dynamics and co-occurrence patterns of honey bee pathogens revealed by high-throughput RT-qPCR analysis
负责人:
关键词:
Apis mellifera;honey bee;Nosema ceranae;pathogens;black queen cell virus;Holocene
DOI:
doi:10.5061/dryad.q7s60q9
摘要:
s for a high-throughput qPCR platform. Assuming that interaction of pathogens requires co-occurrence within the same individual, single worker bees were analyzed
Data from: Extracting DNA from ‘jaws’: high yield and quality from archived tiger shark (Galeocerdo cuvier) skeletal material
负责人:
关键词:
DNA extraction;tiger shark;archived tissue samples;Molecular Biology;DNA capture;Galeocerdo cuvier;Ecological Genetics;population genetics;1912-2015;genomics;qPCR;Laboratory methods;Carcharodon carcharias
DOI:
doi:10.5061/dryad.8080c
摘要:
s (Galeocerdo cuvier). Protocols were compared for DNA yield and quality using a qPCR approach. For jaw swarf, all methods provided relatively high DNA yield and quality
Data from: Early replication dynamics of sex-linked mitochondrial DNAs in the doubly uniparental inheritance species Ruditapes philippinarum (Bivalvia Veneridae)
负责人:
关键词:
doubly uniparental inheritance mtDNA Real-Time qPCR bivalve development
DOI:
doi:10.5061/dryad.q3hs1
摘要:
ng real-time quantitative PCR. We found that both mtDNAs do not detectably replicate during early embryogenesis, and that the M line might be lost from females around 24?h of age
Data from: A novel real-world ecotoxicological dataset of pelagic microbial community responses to wastewater
负责人:
关键词:
DOI:
doi:10.5061/dryad.2fqz612km
摘要:
.? Flow cytometry, amplicon sequencing?of?16S and 18S rRNA genes and micro-fluidic quantitative polymerase-chain reactions (MFQPCR) were used to determine chlorophyll
Data from: Stress hormone receptors change as range expansion progresses in house sparrows
负责人:
关键词:
qPCR;Passer domesticus;glucorticoids
DOI:
doi:10.5061/dryad.8pq6c
摘要:
As ranges expand, individuals encounter different environments at the periphery than at the centre of the range. Previously, we have shown that glucocorticoids (GCs) vary with range expansion: individuals at the range edge release more GCs in response to restraint. Here, we measured hippocampal mRNA expression of GC receptors (mineralocorticoid, MR and glucocorticoid, GR) in eight house sparrow (Passer domesticus) populations varying in age. We found that individuals closest to the range edge had the lowest expression of MR relative to GR; in all likelihood, this relationship was driven by a marginal reduction of MR mRNA at the range edge. Reduced MR (relative to GR) might allow enhanced GC binding to GR, the lower affinity receptor that would enhance a rapid physiological and behavioural response to stressors. The insights gained from this study are not only enlightening to introduced species, but may also predict how certain species will react as their ranges shift owing to anthropogenic changes.
Data from: Individual consumption of supplemental food as a predictor of reproductive performance and viral infection intensity
负责人:
关键词:
stable isotope analysis;BFDV;population recovery;qPCR;supplemental feeding;diet;parrot;reproductive fitness;Psittacula echo
DOI:
doi:10.5061/dryad.t7s15hp
摘要:
d stable isotope analyses to characterise the relative proportional consumption of supplemental food and quantitative polymerase chain reaction (qPCR) to assess beak

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