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Data from: Distance, flow, and PCR inhibition: eDNA dynamics in two headwater steams
- 负责人：
- DOI：
- doi:10.5061/dryad.7j3g5
- 摘要：
- times from mid-summer through autumn, over flows ranging from approximately 1 to 96 L/sec. We used qPCR to relate DNA copy number to distance from source
Data from: Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter
- 负责人：
- DOI：
- doi:10.5061/dryad.p2q4r
- 摘要：
- quantitative PCR (qPCR) and next-generation sequencing (NGS) applications, quality and quantity of the starting material is crucial, calling for optimized capture, storage
Data from: MicroRNA stability in FFPE tissue samples: dependence on GC content
- 负责人：
- 关键词：
- human;MiRNA
- DOI：
- doi:10.5061/dryad.fj0f8
- 摘要：
- in FFPE cardiac tissues instead of miR-1, which was predominant before fixation. Subsequent quantitative PCR (qPCR) analyses revealed that miRNAs
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease
- 负责人：
- DOI：
- doi:10.5061/dryad.3274k
- 摘要：
- disease-relevant changes in miRNA expression. In primary VIC lines from diseased and control valves, miRNA expression was profiled using RT-qPCR
Data from: Effects of prey quality and predator body size on prey DNA detection success in a centipede predator
- 负责人：
- DOI：
- doi:10.5061/dryad.ss17b
- 摘要：
- in PCR-based gut content analysis, potentially resulting in over- or underestimation of feeding rates. Experimental evidence, however, is scarce
Data from: Methodological considerations for detection of terrestrial small-body salamander eDNA and implications for biodiversity conservation
- 负责人：
- DOI：
- doi:10.5061/dryad.tc29r
- 摘要：
- ke management decisions. The objectives of this project were to use quantitative PCR (qPCR) to: (i) detect spiked salamander DNA in soil, (ii) quantify eDNA degradation ove
Data from: Environmental DNA surveillance for invertebrate species: advantages and technical limitations to detect invasive cr
- 负责人：
- DOI：
- doi:10.5061/dryad.0ck3q
- 摘要：
- , the red swamp crayfish Procambarus clarkii. Species-specific primers and probes were designed; their specificity was tested using in silico PCR simulations and ag
Data from: Diet and trophic interactions of a circumglobally significant gelatinous marine zooplankter, Dolioletta gegenbauri (Uljanin, 1884)
- 负责人：
- Frischer, Marc
- 关键词：
- qPCR Doliolid Pelagic Tunicate Selective Feeding South Atlantic Bight Next Generation Sequencing
- DOI：
- doi:10.5061/dryad.99p2308
- 摘要：
- of the South Atlantic Bight (SAB), USA. Sequencing-based approaches were complimented with targeted quantitative real time PCR (Polymerase Chain Reaction) analyses. Captive
Data from: Taxon abundance, diversity, co-occurrence and network analysis of the ruminal microbiota in response to dietary changes in dairy cows
- 负责人：
- Tapio, Ilma
- DOI：
- doi:10.5061/dryad.t6t0q
- 摘要：
- period and DNA was extracted from a combined sample. Diet effect on rumen microbial community was explored by qPCR, T-RFLP and metabarcoding sequencing
Data from: Estimating fish abundance and biomass from eDNA concentrations: variability among capture methods and environmental conditions
- 负责人：
- Anais Lacoursière-Roussel
- DOI：
- doi:10.5061/dryad.46sm5
- 摘要：
- ined by quantitative PCR (qPCR) varied significantly with fish abundance and biomass and type of filters (Mixed-design ANOVA, P < 0.001). Results als
