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Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
- 负责人：
- DOI：
- doi:10.5061/dryad.28n8d6t
- 摘要：
- on in the availability of antibodies for detecting proteins, quantitative PCR (qPCR) remains the preferred method for quantifying target gene knockdown after dsRNA treatment
Data from: Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation
- 负责人：
- DOI：
- doi:10.5061/dryad.1rn8pk0qz
- 摘要：
- and multi-trophic level interactions. Both conventional polymerase chain reaction (cPCR) and quantitative PCR (qPCR) have been employed to detect predati
Data from: The choice of universal primers and the characteristics of the species mixture determines when DNA metabarcoding can be quantitative.
- 负责人：
- DOI：
- doi:10.5061/dryad.q2r3b1f
- 摘要：
- nces the technique can be quantitative. Basically, we simulate a mixture of DNA of S species with a defined initial abundance distribution. In the simulated PCR, ea
Data from: Residual eDNA detection sensitivity assessed by quantitative real-time PCR in a river ecosystem
- 负责人：
- DOI：
- doi:10.5061/dryad.5rf92
- 摘要：
- l eDNA detection sensitivity using quantitative real-time polymerase chain reaction (qRT-PCR), in a flowing, uncontrolled river after the eDNA source
Data from: Attack of the PCR clones: rates of clonality have little effect on RAD-seq genotype calls
- 负责人：
- DOI：
- doi:10.5061/dryad.3mq4631
- 摘要：
- esent in RAD-seq data, quantify how often the presence of clones in a dataset cause genotype calls to change compared to when clones were remove
Data from: A whole-transcriptome approach to evaluating reference genes for quantitative gene expression studies: a case study in Mimulus
- 负责人：
- DOI：
- doi:10.5061/dryad.84655
- 摘要：
- While quantitative PCR (qPCR) is widely recognized as being among the most accurate methods for quantifying gene expression, it is highly dependent
Data from: Environmental DNA analysis as a non-invasive quantitative tool for reproductive migration of a threatened endemic fish in rivers
- 负责人：
- Maruyama, Atsushi
- DOI：
- doi:10.5061/dryad.5hb0fb8
- 摘要：
- (Opsariichthys uncirostris uncirostris), was non-invasively monitored by quantitative PCR of species-specific environmental DNA (eDNA), the usefulness of which ha
Data from: Investigating cat predation as the cause of bat wing tears using forensic DNA analysis
- 负责人：
- DOI：
- doi:10.5061/dryad.zcrjdfn88
- 摘要：
- ng quantitative PCR, cat DNA was found in two-thirds of samples submitted by bat rehabilitators. Of these samples, short tandem repeat analysis produced partial DNA
Data from: Recognizing false positives: synthetic oligonucleotide controls for environmental DNA surveillance
- 负责人：
- DOI：
- doi:10.5061/dryad.h373j
- 摘要：
- . For quantitative PCR (qPCR), false positives in environmental samples can also be detected using a fluorescent probe designed to detect the synthetic insert. The generati
Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods
- 负责人：
- DOI：
- doi:10.5061/dryad.2p51n
- 摘要：
- The quantification of the biological diversity in environmental samples using high-throughput DNA sequencing is hindered by the PCR bias caused
