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Data from: Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding
- 负责人:
- DOI:
- doi:10.5061/dryad.fs728
- 摘要:
- can be countered by (1) targeting loci with highly degenerate primers or conserved priming sites, (2) increasing PCR template concentration, (3) reducing PCR cycle
Data from: Mapping and expression of candidate genes for development rate in rainbow trout (Oncorhynchus mykiss)
- 负责人:
- DOI:
- doi:10.5061/dryad.m4cc1
- 摘要:
- , a Quantitative Trait Locus (QTL) for embryonic development rate has been detected on chromosome 5 across populations. However, few candidate genes have been identi
Data from: Fold or hold: experimental evolution in vitro
- 负责人:
- DOI:
- doi:10.5061/dryad.1nt11
- 摘要:
- quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures tha
Data from: Advanced characterization of DNA molecules in rAAV vector preparations by single-stranded virus next-generation sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.fs4cp
- 摘要:
- studies using quantitative polymerase chain reaction (qPCR) analyses. However, because qPCR only provides a partial view of the DNA molecules in rAAV
Data from: Peripheral blood mitochondrial DNA copy number is associated with prostate cancer risk and tumor burden
- 负责人:
- DOI:
- doi:10.5061/dryad.88896
- 摘要:
- mtDNA copy number was measured using quantitative real-time PCR; samples from three cases and two controls could not be assayed, leaving 193 cases and 194 cont
Data from: Environmental DNA surveys help to identify winter hibernacula of a temperate freshwater turtle
- 负责人:
- DOI:
- doi:10.5061/dryad.3j9kd51d1
- 摘要:
- ), a species of conservation concern in Canada. We collected water samples under the ice in winter across a mid?sized temperate lake and used quantitative PCR
Data from: Multiple-strain infections of borrelia afzelii: a role for within-host interactions in the maintenance of antigenic diversity?
- 负责人:
- DOI:
- doi:10.5061/dryad.24k0s
- 摘要:
- reservoir hosts, the bank vole Myodes glareolus. We measured overall infection intensity with quantitative polymerase chain reaction (PCR) and resolve
Data from: Diet and trophic interactions of a circumglobally significant gelatinous marine zooplankter, Dolioletta gegenbauri (Uljanin, 1884)
- 负责人:
- Frischer, Marc
- 关键词:
- Doliolid Pelagic Tunicate Selective Feeding qPCR South Atlantic Bight Next Generation Sequencing
- DOI:
- doi:10.5061/dryad.99p2308
- 摘要:
- l shelf of the South Atlantic Bight (SAB), USA. Sequencing-based approaches were complimented with targeted quantitative real time PCR (Polymerase Chain Reaction) analyses. Captive
Data from: Environmental DNA surveillance for invertebrate species: advantages and technical limitations to detect invasive cr
- 负责人:
- DOI:
- doi:10.5061/dryad.0ck3q
- 摘要:
- ainst tissues of other crayfish species. Limits of detection and quantification were specified for the target DNA sequence by means of quantitative PCR amplificati
Data from: Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish
- 负责人:
- DOI:
- doi:10.5061/dryad.2f681
- 摘要:
- r ability to detect and quantify common carp (Cyprinus carpio) mitochondrial DNA using quantitative PCR in a series of laboratory experiments. Filtrati