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Data from: Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding
负责人:
关键词:
Arthropoda;Arthropods
DOI:
doi:10.5061/dryad.fs728
摘要:
can be countered by (1) targeting loci with highly degenerate primers or conserved priming sites, (2) increasing PCR template concentration, (3) reducing PCR cycle
Data from: Mapping and expression of candidate genes for development rate in rainbow trout (Oncorhynchus mykiss)
负责人:
关键词:
quantitative genetics;Quantitative genetics and Mendelian inheritance;qPCR;quantitative trait loci;salmonid Subject area: Genomics and gene mapping
DOI:
doi:10.5061/dryad.m4cc1
摘要:
, a Quantitative Trait Locus (QTL) for embryonic development rate has been detected on chromosome 5 across populations. However, few candidate genes have been identi
Data from: Fold or hold: experimental evolution in vitro
负责人:
关键词:
molecular evolution;Synthetic short oligonucleotides (Oli populations);Simulation;Experimental evolution;Adaptation
DOI:
doi:10.5061/dryad.1nt11
摘要:
quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures tha
Data from: Advanced characterization of DNA molecules in rAAV vector preparations by single-stranded virus next-generation sequencing
负责人:
关键词:
Contaminants;Illumina;Adeno-associated virus;quality control;quantitative PCR;AAV;DNA;bioinformatics;gene therapy;vector;NGS;virus
DOI:
doi:10.5061/dryad.fs4cp
摘要:
studies using quantitative polymerase chain reaction (qPCR) analyses. However, because qPCR only provides a partial view of the DNA molecules in rAAV
Data from: Peripheral blood mitochondrial DNA copy number is associated with prostate cancer risk and tumor burden
负责人:
关键词:
human;prostate cancer;Mitochondrial DNA;cancer risk;Homo Sapiens;tumor burden
DOI:
doi:10.5061/dryad.88896
摘要:
mtDNA copy number was measured using quantitative real-time PCR; samples from three cases and two controls could not be assayed, leaving 193 cases and 194 cont
Data from: Environmental DNA surveys help to identify winter hibernacula of a temperate freshwater turtle
负责人:
关键词:
Environmental DNA;Graptemys geographica;Northern map turtle;overwintering;quantitative real\u2010time PCR
DOI:
doi:10.5061/dryad.3j9kd51d1
摘要:
), a species of conservation concern in Canada. We collected water samples under the ice in winter across a mid?sized temperate lake and used quantitative PCR
Data from: Multiple-strain infections of borrelia afzelii: a role for within-host interactions in the maintenance of antigenic diversity?
负责人:
关键词:
competition;Immunity: ecology;bacteria;Coevolution;Immunity: evolution
DOI:
doi:10.5061/dryad.24k0s
摘要:
reservoir hosts, the bank vole Myodes glareolus. We measured overall infection intensity with quantitative polymerase chain reaction (PCR) and resolve
Data from: Diet and trophic interactions of a circumglobally significant gelatinous marine zooplankter, Dolioletta gegenbauri (Uljanin, 1884)
负责人:
Frischer, Marc
关键词:
Doliolid Pelagic Tunicate Selective Feeding qPCR South Atlantic Bight Next Generation Sequencing
DOI:
doi:10.5061/dryad.99p2308
摘要:
l shelf of the South Atlantic Bight (SAB), USA. Sequencing-based approaches were complimented with targeted quantitative real time PCR (Polymerase Chain Reaction) analyses. Captive
Data from: Environmental DNA surveillance for invertebrate species: advantages and technical limitations to detect invasive cr
负责人:
关键词:
efficiency of detection;funnel traps;qPCR;Procambarus clarkii;eDNA;limit of detection;limit of quantification;biological invasions
DOI:
doi:10.5061/dryad.0ck3q
摘要:
ainst tissues of other crayfish species. Limits of detection and quantification were specified for the target DNA sequence by means of quantitative PCR amplificati
Data from: Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish
负责人:
关键词:
Conservation Biology Environmental DNA Fish Fisheries Management Invasive Species
DOI:
doi:10.5061/dryad.2f681
摘要:
r ability to detect and quantify common carp (Cyprinus carpio) mitochondrial DNA using quantitative PCR in a series of laboratory experiments. Filtrati

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