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共检索到71条 ,权限内显示50条;
Data from: Environmental DNA analysis of river herring in Chesapeake Bay: a powerful tool for monitoring threatened keystone species
- 负责人：
- DOI：
- doi:10.5061/dryad.n60t8b3
- 摘要：
- e developed and field-tested a qPCR assay to detect eDNA from alewife and blueback herring (collectively known as ‘river herring’), comparing eDNA-based
Data from: Long-range PCR allows sequencing of mitochondrial genomes from environmental DNA
- 负责人：
- DOI：
- doi:10.5061/dryad.q5gg0
- 摘要：
- ny problems associated with identification of species from short-fragment PCR amplicon-based methods.
Data from: Detection of invasive mosquito vectors using environmental DNA (eDNA) from water samples
- 负责人：
- 关键词：
- Aedes albopictus;Culicidae;Aedes koreicus;detection probability;vector;eDNA;Aedes japonicus;mosquito
- DOI：
- doi:10.5061/dryad.22782
- 摘要：
- h molecular methods, confirming the reliability and efficiency of alternative eDNA-based techniques for the early and unambiguous detection and surveillance of invasive
Data from: Phylogenetic affiliation of SSU rRNA genes generated by massively parallel sequencing: new insights into the freshwater protist diversity
- 负责人：
- 关键词：
- Protozoa;protist
- DOI：
- doi:10.5061/dryad.66hc3
- 摘要：
- t of this work, a tree-based method was compared with different approaches of taxonomic affiliation (BLAST and RDP) of 18S rRNA gene sequences and was shown to be the mos
Data from: ‘True’ null allele detection in microsatellite loci: a comparison of methods, assessment of difficulties, and survey of poss
- 负责人：
- DOI：
- doi:10.5061/dryad.s571g
- 摘要：
- and to provide improvements. We applied several approaches to identify the ‘true’ null alleles based on the predictions made by five different methods, used either
Data from: Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae
- 负责人：
- Raquin Vincent
- DOI：
- doi:10.5061/dryad.pm71k02
- 摘要：
- animal physiology, development or host-microbe interactions. While adult flies can be easily assigned to a given Drosophila species based
Data from: Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis
- 负责人：
- DOI：
- doi:10.5061/dryad.qb4gs
- 摘要：
- and benchmarking of phenetic classifications, and finally to develop a hierarchical PCR-based typing scheme based on single nucleotide polymorphism sites (SNPs
Data from: ITS1: a DNA barcode better than ITS2 in eukaryotes?
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.n56t9
- 摘要：
- , and 19,060 species, were analyzed. Using similarity-based methods, we calculated species discrimination efficiencies for ITS1 and ITS2 in all major groups
Data from: Estimating fish abundance and biomass from eDNA concentrations: variability among capture methods and environmental conditions
- 负责人：
- Anais Lacoursière-Roussel
- DOI：
- doi:10.5061/dryad.46sm5
- 摘要：
- the importance of adequate filters to quantify fish abundance based on the eDNA method. This study supports the importance of including water temperature
Data from: Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment
- 负责人：
- 关键词：
- eukaryotes;ecosystem based management;biodiversity;prokaryotes;Environmental DNA;shotgun sequencing
- DOI：
- doi:10.5061/dryad.qq11c
- 摘要：
- of this method for assessing eukaryotic diversity. In contrast, using ‘tree of life’ (ToL) metabarcoding and 20-fold fewer sequencing reads, we could detect 287
