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Data from: PCR duplication: a one-step cloning-free method to generate duplicated chromosomal loci and interference-free expression reporters
- 负责人：
- DOI：
- doi:10.5061/dryad.gj51n
- 摘要：
- s in the yeast genome using a simple PCR-based approach. To demonstrate its application we first duplicated the promoter of the FAR1 gene in yeast and simultaneously
Data from: Improving PCR detection of prey in molecular diet studies: importance of group-specific primer set selection and extraction protocol
- 负责人：
- Zarzoso-Lacoste, Diane
- 关键词：
- Invasive rats Diet Analysis PCR-based method Extraction protocol performances A260/A280 absorbance ratio Group-specific primer pairs
- DOI：
- doi:10.5061/dryad.8c134
- 摘要：
- indicate that this method does not detect all consumed prey. Polymerase Chain Reaction based methods are increasingly used to detect prey DNA
Data from: Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding
- 负责人：
- DOI：
- doi:10.5061/dryad.fs728
- 摘要：
- can be derived from metabarcoding due to taxon specific PCR amplification biases. PCR-free approaches have been suggested to mitigate this problem
Data from: Direct sequencing of haplotypes from diploid individuals through a modified emulsion PCR-based single-molecule sequencing approach
- 负责人：
- 关键词：
- Eurtytemora affinis
- DOI：
- doi:10.5061/dryad.rh7f4
- 摘要：
- , we developed a simple PCR-based method for obtaining sequence information from individual DNA strands using standard laboratory equipment. The method employs
Data from: Optimizing methods for PCR-based analysis of predation
- 负责人：
- DOI：
- doi:10.5061/dryad.8947
- 摘要：
- d in significantly increased prey DNA detection rates in both predator taxa. Among the three post-PCR visualization methods, an eightfold difference
Data from: Targeted gene enrichment and high-throughput sequencing for environmental biomonitoring: a case study using freshwater macroinvertebrates
- 负责人：
- DOI：
- doi:10.5061/dryad.67n7n
- 摘要：
- s. The data from this study illustrate the challenges of using PCR amplification-based methods for biomonitoring and highlight the potential benefits of usi
Data from: Clinical and laboratory predictors of influenza infection among individuals with influenza-like illness presenting to an urban Thai
- 负责人：
- DOI：
- doi:10.5061/dryad.t7n48
- 摘要：
- . Vaccine effectiveness (VE) was calculated using the case test-negative method. Classification and Regression Tree (CART) analysis was used to predict influenza RT-PCR
Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.
- 负责人：
- Brelsford, Alan
- DOI：
- doi:10.5061/dryad.1hg75
- 摘要：
- , and library preparation methods based on the AFLP technique may be particularly susceptible. Here, we compare AFLP profiles generated with standard Taq
Data from: “Direct PCR” optimization yields a rapid, cost-effective, non-destructive, and efficient method for obtaining DNA barcodes without DNA extraction
- 负责人：
- DOI：
- doi:10.5061/dryad.4q4kf
- 摘要：
- PCR method that skips DNA extraction (“direct PCR”) and that can be used for a broad range of invertebrate taxa. We demonstrate how direct PCR can be optimized
Data from: Mitochondrial capture enriches mito-DNA 100 folds enabling PCR-free mitogenomics biodiversity analysis
- 负责人：
- DOI：
- doi:10.5061/dryad.19nf6
- 摘要：
- Biodiversity analyses based on Next Generation Sequencing (NGS) platforms have developed by leaps and bounds in recent years. A PCR-free strategy
