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Data from: 18S rRNA V9 metabarcoding for diet characterization: a critical evaluation with two sympatric zooplanktivorous fish species
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.2p1t8
- 摘要：
- . The high sensitivity and semi-quantitative nature of the 18S V9 metabarcoding approach was supported by both visual microscopy and qPCR-based result
Data from: Mining from transcriptomes: 315 single-copy orthologous genes concatenated for the phylogenetic analyses of Orchidaceae
- 负责人：
- DOI：
- doi:10.5061/dryad.qm30m
- 摘要：
- on morphology and subjective factors. Although more reliable than classic phylogenic analyses, the current methods are based on a few gene markers
Data from: Finding flies in the mushroom soup: host specificity of fungus-associated communities revisited with a novel molecular method
- 负责人：
- DOI：
- doi:10.5061/dryad.tf56fm1
- 摘要：
- t molecular methods are suitable for studying this rich natural microcosm. Comparing to previous knowledge based on rearing and morphology-based identifications
Data from: Minimizing polymerase biases in metabarcoding
- 负责人：
- DOI：
- doi:10.5061/dryad.k129c
- 摘要：
- simultaneously amplify a short, variable genomic region, or “barcode”, from a broad taxonomic group via the polymerase chain reaction (PCR), usi
Data from: Whole transcriptome RNA-Seq analysis of breast cancer recurrence risk using formalin-fixed paraffin-embedded tumor tissue
- 负责人：
- DOI：
- doi:10.5061/dryad.q760h
- 摘要：
- RNA biomarkers discovered by RT-PCR-based gene expression profiling of archival formalin-fixed paraffin-embedded (FFPE) tissue form the bas
Data from: Benefits and challenges with applying unique molecular identifiers in next generation sequencing to detect low frequency mutations
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.n6068
- 摘要：
- mutations, as true mutations could be distinguished from PCR errors or sequencing errors based on consensus among reads sharing same index. In an effort
Data from: Sex determination in the wild: a field application of loop-mediated isothermal amplification successfully determines sex across thr
- 负责人：
- Centeno-Cuadros, Alejandro
- 关键词：
- Loop-mediated isothermal amplification CHD-W CHD-Z Birds landscape genetics wildlife management ecological genetics
- DOI：
- doi:10.5061/dryad.4kr93
- 摘要：
- PCR-based methods are the most common technique for sex determination of birds. Although these methods are fast, easy and accurate, they sti
Data from: DNA metabarcoding for high-throughput monitoring of estuarine macrobenthic communities
- 负责人：
- DOI：
- doi:10.5061/dryad.2686c
- 摘要：
- quently, we compared morphology and metabarcoding-based approaches to determine the species composition from four distinct estuarine sites. Our results indicate tha
Data from: Trace DNA from insect skins: a comparison of five extraction protocols and direct PCR on chironomid pupal exuviae
- 负责人：
- DOI：
- doi:10.5061/dryad.tf6r4
- 摘要：
- and other cell-based structures might accidentally attach as they are shed. This source of trace DNA can be sufficient for PCR amplification and se
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
- 负责人：
- DOI：
- doi:10.5061/dryad.n4cv3
- 摘要：
- The formation of chimeric sequences can create significant methodological bias in PCR-based DNA metabarcoding analyses. During mixed-template
