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Data from: A new plant virus discovered by immunocapture of double stranded RNA; assessment of a novel approach for viral metagenomics studies
- 负责人：
- Blouin, Arnaud G.
- 关键词：
- Metagenomics antibodies dsRNA NGS virus
- DOI：
- doi:10.5061/dryad.0bg04
- 摘要：
- decrease in sequencing costs, it is difficult to justify their use in large-scale surveys without a virus sequence enrichment technique. As the majority
Data from: The power and promise of RNA-seq in ecology and evolution
- 负责人：
- 关键词：
- biological replication differential expression experimental design power analysis RNA sequencing Transcriptomics
- DOI：
- doi:10.5061/dryad.vp42s
- 摘要：
- of technology and experimental design may be initially neglected. Here, we review these issues with regard to RNA sequencing (RNA-seq). RNA-seq adds large-scale
Data from: Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian
- 负责人：
- DOI：
- doi:10.5061/dryad.179116t
- 摘要：
- s. Molecular analyses of these gene families require high-confidence sequences, but the high sequence similarity of the members can create challenges for sequencing
Data from: Ion Torrent PGM as tool for fungal community analysis: a case study of endophytes in Eucalyptus grandis reveals high taxonomic diversity
- 负责人：
- DOI：
- doi:10.5061/dryad.51rf8
- 摘要：
- . This is especially true for endophytic communities of fungi living in healthy plant tissue. The developments in next generation sequencing technologies ar
Data from: High-throughput sequencing of nematode communities from total soil DNA extractions
- 负责人：
- DOI：
- doi:10.5061/dryad.0h653
- 摘要：
- alternative. Generally, primers that have been used for generating amplicons for sequencing are not nematode specific and also amplify other groups suc
Data from: The complete sequence of the mitochondrial genome of Butomus umbellatus - a member of an early branching lineage of monocotyledons
- 负责人：
- DOI：
- doi:10.5061/dryad.42gc4
- 摘要：
- of Butomus umbellatus (Butomaceae) based on next-generation sequencing data. The genome was assembled into a circular molecule, 450,826 bp in length. Coding sequences
Data from: Bacterial characterization of Beijing drinking water by flow cytometry and MiSeq sequencing of the 16S rRNA gene
- 负责人：
- DOI：
- doi:10.5061/dryad.55nb0
- 摘要：
- Flow cytometry (FCM) and 16S rRNA gene sequencing data are commonly used to monitor and characterize microbial differenc
Data from: Unmapped sequencing reads identify additional candidate genes linked to magnetoreception in rainbow trout
- 负责人：
- DOI：
- doi:10.5061/dryad.4g048
- 摘要：
- ng the available sequencing data from the trout brain transcriptomes, we assembled >27 million unmapped sequences (5.8% of total sequences) into 45,142 contigs
Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.
- 负责人：
- Brelsford, Alan
- DOI：
- doi:10.5061/dryad.1hg75
- 摘要：
- nt for sequencing applications, we suggest that it may be more effective to use alternative library preparation methods.
Data from: The effects of read length, quality and quantity on microsatellite discovery and primer development: from Illumina to PacBio
- 负责人：
- DOI：
- doi:10.5061/dryad.r20t0
- 摘要：
- luding single-molecule real-time DNA sequencing (Pacific Biosciences, or PacBio) remain viable alternatives. We outline a workflow from sequence quality control
