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Data from: Inferring the mode of origin of polyploid species from next-generation sequence data
负责人:
关键词:
approximate Bayesian computation;Capsella;Polyploidization;Disomic inheritance;speciation
DOI:
doi:10.5061/dryad.g611t
摘要:
es on the basis of both old and new sequencing technologies. Application of ABC to sequence data from diploid and polyploid species of the plant genus Capse
Data from: Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community
负责人:
关键词:
Fungi;Fluidigm;Mock Community;ITS;Primer Selection;LSU
DOI:
doi:10.5061/dryad.tn6vn
摘要:
With the increasing democratization of high-throughout sequencing (HTS) technologies, along with the concomitant increase in sequence yield pe
Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar
负责人:
关键词:
Illumina MiSeq;microfluidic PCR;genome-tagged amplification;Miocene;Burseraceae;targeted amplicon sequencing;Commiphora;phylogenomics;Bursera
DOI:
doi:10.5061/dryad.591q3
摘要:
to maximize sequencing depth efficiency. The principle benefit of microfluidic PCR over alternative target enrichment strategies is that it bypasses costly
Data from: High-throughput amplicon sequencing of rRNA genes requires a copy number correction to accurately reflect the effect
负责人:
关键词:
burning;metagenomics;Nitrogen enrichment;Konza prairie;tallgrass prairie;nematodes
DOI:
doi:10.5061/dryad.t8g16
摘要:
frequencies based on amplicon sequencing were not initially consistent with specimen-based counts, but correction for differences in rRNA gene copy number usi
Data from: Sequence capture phylogenomics of historical ethanol-preserved museum specimens: unlocking the rest of the vault
负责人:
关键词:
museum collections;degraded DNA;Opiliones;ultraconserved elements;ethanol preservation;phylogenomics
DOI:
doi:10.5061/dryad.sh5b962
摘要:
museum specimens with highly degraded DNA, which until recently have been deemed less valuable for genetic work. The successful sequencing of ultraconse
Data from: Efficient detection of novel nuclear markers for Brassicaceae by transcriptome sequencing
负责人:
关键词:
Primer-design;Arabidopsis thaliana;primer database;Next-generation sequencing;RNA-seq;phylogenetics;Cardamine hirsuta;Arabis alpina;454 transcriptome sequencing;Brassicaceae
DOI:
doi:10.5061/dryad.63j2j
摘要:
of molecular polymorphisms in nuclear markers. Next-generation sequencing (NGS) techniques nowadays provide a powerful approach to overcome this limitation. We present
Data from: MicroRNA stability in FFPE tissue samples: dependence on GC content
负责人:
关键词:
human;MiRNA
DOI:
doi:10.5061/dryad.fj0f8
摘要:
and deep sequencing, are performed in order to identify miRNA biomarkers, using archival patient tissue samples. MiRNAs are more robust than longer RNAs, and r
Data from: Phylogenetic affiliation of SSU rRNA genes generated by massively parallel sequencing: new insights into the freshwater protist diversity
负责人:
关键词:
Protozoa;protist
DOI:
doi:10.5061/dryad.66hc3
摘要:
by highlighting, for example, the rare biosphere. Currently, high-throughput pyrotag sequencing of PCR-amplified SSU rRNA gene regions is mainly use
Data from: Immunomics of the koala (Phascolarctos cinereus )
负责人:
关键词:
annotation;koala retrovirus;spleen;vertebrate;mRNA;Major histocompatibility complex;ribosomal RNA;San Diego Zoo koala colony;RIG-I like receptor;buffy coat;cDNA;Phascolarctos cinereus;Toll-like receptor
DOI:
doi:10.5061/dryad.sc6bp
摘要:
antigens—and to help combat infectious diseases (e.g., chlamydiosis) that impede ongoing conservation efforts. We used Illumina sequencing of cDNA

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