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Data from: Not all sequence tags are created equal: designing and validating sequence identification tags robust to indels
- 负责人：
- DOI：
- doi:10.5061/dryad.4m0v8474
- 摘要：
- Ligating adapters with unique synthetic oligonucleotide sequences (sequence tags) onto individual DNA samples before massively parallel sequencing
Data from: The QRS (Quantification of Representative Sequences) pipeline for amplicon sequencing: case study on within-population ITS1
- 负责人：
- DOI：
- doi:10.5061/dryad.v7f7r
- 摘要：
- d by Sanger sequencing of cloned PCR products. This was performed by analysing sequence variation of the highly variable first internal transcribed spacer (ITS1
Data from: Efficient in situ barcode sequencing using padlock probe-based BaristaSeq
- 负责人：
- 关键词：
- in situ sequencing
- DOI：
- doi:10.5061/dryad.5dd92
- 摘要：
- approaches to reading out cellular barcodes trade off spatial resolution with throughput. Bulk sequencing achieves high throughput but sacrifices
Data from: BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand speci
- 负责人：
- DOI：
- doi:10.5061/dryad.9mq14
- 摘要：
- Next Generation Sequencing (NGS) is driving rapid advancement in biological understanding and RNA-sequencing (RNA-seq) has become an indispensable
Data from: Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix)
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.n8v4v6d
- 摘要：
- for amplicon sequencing on Illumina instruments. In the variant we use most frequently for large-scale projects, we fuse partial adapter sequences (TruSeq or Nextera) ont
by next-generation sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.1924
- 摘要：
- ome an extremely valuable tool for the study of this process by allowing whole transcriptome sequencing, or RNA-Seq. We have conducted a 454 GS-FLX pyrosequencin
Data from: The phylogenetic utility and functional constraint of microRNA flanking sequences
- 负责人：
- DOI：
- doi:10.5061/dryad.49q24
- 摘要：
- . As next-generation sequencing makes it more cost-effective to sequence genomes and small RNA libraries, this methodology provides an alternative data source
Data from: Species level phylogeny and polyploid relationships in Hordeum (Poaceae) inferred by next-generation sequencing and in-silico cloning
- 负责人：
- DOI：
- doi:10.5061/dryad.fn2nt
- 摘要：
- ed in a single run on a 454 platform. Reference sequences were obtained by cloning and Sanger sequencing of all loci for nine supplementary individuals. The 454
Data from: Small RNAs from a big genome: the piRNA pathway and transposable elements in the salamander species Desmognathus fuscus
- 负责人：
- Mueller, Rachel L.
- 关键词：
- genome size evolution small RNA evolution transposable element silencing piRNA pathway salamanders vertebrates
- DOI：
- doi:10.5061/dryad.kv57r
- 摘要：
- of the ancestral piRNA-mediated TE-silencing machinery. We characterized the small RNA pool in the female and male adult gonads, testing for the presenc
