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Data from: Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from single specimens
- 负责人:
- DOI:
- doi:10.5061/dryad.p25vn
- 摘要:
- ts, inferior to next-generation sequencers, which are capable of producing millions of sequence reads simultaneously. Additionally, direct Sanger sequencing
Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.
- 负责人:
- Brelsford, Alan
- DOI:
- doi:10.5061/dryad.1hg75
- 摘要:
- High-fidelity “proofreading” polymerases are often used in library construction for next-generation sequencing projects, in an effort to minimize
Data from: Fast and cost-effective genetic mapping in apple using next-generation sequencing
- 负责人:
- 关键词:
- Malus x domestica
- DOI:
- doi:10.5061/dryad.55t54
- 摘要:
- Next-generation DNA sequencing (NGS) produces vast amounts of DNA sequence data, but it is not specifically designed to generate data suitable
Data from: The QRS (Quantification of Representative Sequences) pipeline for amplicon sequencing: case study on within-population ITS1
- 负责人:
- DOI:
- doi:10.5061/dryad.v7f7r
- 摘要:
- Next generation sequencing (NGS) platforms are replacing traditional molecular biology protocols like cloning and Sanger sequencing. However
Data from: Unforeseen consequences of excluding missing data from next-generation sequences: simulation study of RAD sequences
- 负责人:
- DOI:
- doi:10.5061/dryad.jf361
- 摘要:
- simulations, focusing specifically on RAD sequences, to highlight some of the unforeseen consequence of excluding missing data from next-generation sequencing
Data from: Using Illumina Next Generation Sequencing Technologies to sequence multigene families in de novo species
- 负责人:
- DOI:
- doi:10.5061/dryad.20r9k
- 摘要:
- The advent of Next Generation Sequencing Technology (NGST) has revolutionized molecular biology research, allowing for rapid gene/genome sequencing
Data from: A first look at the Oxford Nanopore MinION sequencer
- 负责人:
- DOI:
- doi:10.5061/dryad.5p0c3
- 摘要:
- Oxford Nanopore's third-generation, single-molecule sequencing platform promises to decrease costs for reagents and instrumentation. After a two-yea
Data from: Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: comparison
- 负责人:
- DOI:
- doi:10.5061/dryad.3447g
- 摘要:
- Objective: To validate the targeted next-generation sequencing (NGS) platform-Ion Torrent PGM for KRAS exon 2 and expanded RAS mutations detecti
Data from: Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies
- 负责人:
- DOI:
- doi:10.5061/dryad.6fm1p
- 摘要:
- During the past decade, DNA sequencing output has been mostly dominated by the second generation sequencing platforms which are character
Data from: From benchtop to desktop: important considerations when designing amplicon sequencing workflows
- 负责人:
- DOI:
- doi:10.5061/dryad.2qf0t
- 摘要:
- samples and it is therefore imperative that careful attention is given to sample preparation and library generation within workflows, especially those