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ng next generation sequencing
负责人:
关键词:
Next-generation sequencing;cell-free DNA;Melanoma;digital droplet PCR;pancreatic ductal adenocarcinoma;colorectal adenocarcinoma
DOI:
doi:10.5061/dryad.7pj51v0
摘要:
next-generation sequencing panel, variant alleles were on average 2-fold enriched in the short fraction (median insert size: ~142 bp) compared to the original ccfDNA sample
Data from: Rapid microsatellite marker development for African mahogany (Khaya senegalensis, Meliaceae) using next-generation sequencing
负责人:
关键词:
conservation genetics;Biotechnology;Khaya senegalensis;phylogeography;Cedreloideae;Meliaceae;Population Genetics - Empirical
DOI:
doi:10.5061/dryad.s03t592c
摘要:
breeding programs and effective conservation management of the species. Next generation sequencing yielded 93 943 sequences with an average read length of 234 bp
Data from: Revisiting comparisons of genetic diversity in stable and declining species: assessing genome-wide polymorphism in North Amer
负责人:
关键词:
conservation genetics;Bumble bee;Genomics\/Proteomics;insects;Bombus pensylvanicus;Bombus impatiens;RADtag;genetic diversity;Population Genetics - Empirical
DOI:
doi:10.5061/dryad.12j05
摘要:
these discrepancies and to investigate the best ways to integrate next-generation sequencing data and more traditional molecular markers in studies
Data from: Benefits and challenges with applying unique molecular identifiers in next generation sequencing to detect low frequency mutations
负责人:
关键词:
DOI:
doi:10.5061/dryad.n6068
摘要:
sampling bias, rather than PCR errors, challenges the UID-deep sequencing method in faithfully detecting low frequency mutation.
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms
负责人:
关键词:
Genomics\/Proteomics;Natural Selection and Contemporary Evolution;phylogeography;molecular evolution;quantitative genetics;Ecological Genetics
DOI:
doi:10.5061/dryad.7tb72
摘要:
library. We filtered the genomic data using Stacks, a bioinformatic tool to process RAD sequencing data. On average, we obtained ~11 000 polymorphic loci per
Data from: Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian
负责人:
关键词:
Genomics\/Proteomics;targeted sequence capture;Transcriptomics;Mammals;olfactory receptor;multigene family
DOI:
doi:10.5061/dryad.179116t
摘要:
s. Molecular analyses of these gene families require high-confidence sequences, but the high sequence similarity of the members can create challenges for sequencing
Data from: Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms
负责人:
关键词:
DNA Barcoding;metagenomics;Bacillariophyta;diatoms;Algae;Community Ecology
DOI:
doi:10.5061/dryad.370nq
摘要:
The recent emergence of barcoding approaches coupled to those of Next Generation Sequencing (NGS) have raised new perspectives for studying
Data from: Lessons learned from microsatellite development for non-model organisms using 454 pyrosequencing
负责人:
关键词:
Comparative studies;shotgun sequencing;next generation sequencing technology;population genetics;massively parallel sequencing;conservation genetics
DOI:
doi:10.5061/dryad.22p7m
摘要:
. Next Generation Sequencing techniques such as 454 pyrosequencing allow the rapid development of microsatellite markers in nonmodel organisms. 454 pyrosequencin
Data from: Utilizing RADseq data for phylogenetic analysis of challenging taxonomic groups: a case-study in Carex sect. Racemosae
负责人:
关键词:
Glaciation;Next-generation sequencing;SVDQuartets;Cyperaceae;Miocene to Holocene;Carex;Pleistocene
DOI:
doi:10.5061/dryad.4b8j7
摘要:
h Sanger sequencing and next-generation RADseq data sets to estimate phylogenetic relationships among species of Carex section Racemosae (Cyperaceae), a clade large
Data from: Genomics of Compositae weeds: EST libraries, microarrays, and evidence of introgression
负责人:
Grassa, Chris
关键词:
ESTs hybridization introgression invasive plants microarray development next generation sequencing sequence assemblies transcriptome sequencing weeds
DOI:
doi:10.5061/dryad.cm7td
摘要:
and Helianthus, but not in Ambrosia and Lactuca. CONCLUSIONS: Transcriptome sequencing using next generation platforms has greatly reduced the cost of ge

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