The invention provides a preparation for preparing a composition for simulating a sports effect by using bitter gourd seed oil, which can suppress appetite, promote protein production of mitochondria and cytochrome C (CytC) in muscle, and can achieve an adjustment body. The function of fat also has the function of improving exercise endurance; thereby, bitter gourd seed oil can be applied as a composition for simulating exercise effects, and can be applied to various functional health foods or drinks in various dosage forms. It is intended for users to take, and because bitter gourd seed oil is taken from natural sources, it does not burden the human body's cardiovascular and liver, and can reduce the consumer's doubts about product safety.本發明提供一種使用苦瓜籽油製備用以模擬運動效果之組合物之用途,苦瓜籽油能夠抑制食慾、促進肌肉中之粒線體及細胞色素C(CytC)的蛋白質生成,同時能夠達到調整體脂肪之作用,除此之外還具有提升運動耐力之功能;藉此,苦瓜籽油可以運用於作為模擬運動效果之組合物,進而能夠以多種不同劑型形式應用於各種機能性的保健食品或飲品中供使用者服用,並且因苦瓜籽油取自天然來源,不會對人體心血管及肝臟造成負擔,能夠降低服用者對於產品安全之疑慮。