The invention relates to an application of a bitter melon seed oil and a composition thereof. The bitter melon seed oil has an anti-aging capability. Therefore the bitter melon seed oil can be used to manufacture compositions for anti-aging.本發明提供一種苦瓜籽油之用途及基於此用途的組合物。所述苦瓜籽油含有有效劑量之共軛次亞麻油酸,且所述苦瓜籽油具有抗老化之功效。藉此,所述苦瓜籽油可用於製備抗老化的組合物。