New viral genome construction of a recombinant Marek's disease virus, comprising fragments as their identification markers of two fragments, useful for the preparation of vaccines against the Marek's disease virus
SHANDONG AGRICULTURAL UNIVERSITY;SHANDONG AGRICULTURAL UNIVERSITY
发明人:
CUI ZHI-ZHONG,ZHI-ZHONG CUI,SU SHUAI,SHUAI SU,ZHAO PENG,PENG ZHAO
申请号：
FR1161641
公开号：
FR2968672A1
申请日:
2011.12.14
申请国别(地区):
FR
年份:
2012
代理人:
摘要：
Viral genome construction of a recombinant Marek's disease virus, comprising fragments as their identification markers: (1) two fragments of 34 base pairs (bp) having SEQ ID NO: 5 resulting from deletion of the Meq gene of the two original sites containing Meq gene； and (2) a fragment of about 539 bp of the long terminal repetition of the reticuloendotheliosis virus (REV-LTR) having SEQ ID NO: 6 inserted to 370 bp in upstream of the junction site US/TRS in the viral genome, is new. Viral genome construction of a recombinant Marek's disease virus, comprising fragments as their identification markers: (1) two fragments of 34 bp having SEQ ID NO: 5 (comprising a fully defined 34 base pair sequence as given in the specification) resulting from deletion of the Meq gene of the two original sites containing Meq gene； and (2) a fragment of about 539 bp of the long terminal repetition of the reticuloendotheliosis virus (REV-LTR) having SEQ ID NO: 6 (a fully defined 539 base pair sequence as given in the specification) inserted to 370 bp in upstream of the junction site US/TRS in the viral genome, is new. Independent claims are included for: (1) SC9-1 or SC9-2 vaccine strain of the recombinant Marek's disease virus comprising the genome construction, where the SC9-1 strain of MDV is filed at the General center of collection of microbiological cultures of China on December 3, 2010, with deposit number CGMCC NO: 4399, and SC9-2 strain of MDV is filed at the General center of collection of microbiological cultures China on November 24, 2011 with the deposit number CGMCC NO: 5502； (2) a chicken embryo fibroblasts, which are infected by the strain SC9-1 or SC9-2 of recombinant MDV； (3) a vaccine against Marek's disease virus, comprising the genome construction of recombinant Marek's disease virus； (4) a process of preventing Marek's disease in chickens, the chickens receive effective prophylactic doses of vaccines against Marek's disease containing the genome construction of recom
