DR SUBRATA SINHA,DR BIPLAB BOSE,DR NAVIN KHANNA,DR SUBRAT KUMAR ACHARYA
申请号：
IN2704/DEL/2006
公开号：
IN251904B
申请日:
2006.12.18
申请国别(地区):
IN
年份:
2012
代理人:
摘要：
This invention relates to a recombinant chimeric Fab antibody comprising a recombinant Fd having seq id# 1 and a recombinant chimeric light chain having seq id# 2 which binds to Hepatitis B surface antigen (HBsAg) with high affinity {KD= 8.166 ± 0.14 nmol/L), generated by fusing heavy chain variable region (VH) of anti-HBsAg antibody 5S having seq id# 3 with first constant region (CHI) of human IgGl and light chain variable region (VL) of anti-HBsAg antibody 5S having seq id# 4 with constant region (CL) of human kappa chain, wherein mouse VL and human CI are linked by overlap PCR to generate chimeric light chain and mouse VH and CH1 are linked by overlap PCR to generate chimeric Fd using such primers as herein described. Supportive of their active, and folded nature and stabilize the same. This is through the sh groups present on the amino acid present on the amino acid entertain disulphide bond molecules. There are numerous cysteins in each of the chains, most form intra chain disulphide bond. However there is only
