PURPOSE: A health functional composition containing a Corylus heterophylla Fisch. ex Trautv. pollen extract is provided to ensure anti-inflammatory, antioxidative, and antibacterial effects.CONSTITUTION: A method for preparing a health functional composition with anti-inflammatory, antioxidative, and antibacterial effects comprises: a step of dissolving dry powder of Corylus heterophylla Fisch. ex Trautv. in 70% ethanol and extracting and filtering at room temperature to prepare a crude extract a step of dissolving the crude extract in 20% methanol and extracting with hexane to prepare a hexane fraction a step of extracting the hexane fraction-removed residue with dichloromethane to prepare a dichloromethane fraction a step of extracting dichloromethane fraction-removed residue with ethyl acetate to prepare an ethyl acetate fraction a step of extracting the ethyl acetate fraction-removed residue with butanol to prepare a butanol fraction and water fraction.COPYRIGHT KIPO 2013[Reference numerals] (1) Extraction with 70% EtOH (2) Stirring at room temperature for 24 hours (3) Vacuum filtration (AA) Dry powder(600g) (BB) Extraction with 70% EtOH(185g) (CC) Fractionating by separately storing 35g and using 150g (DD) Suspension of 20% MeOH (EE) Extraction with hexane(1:1) (FF) Extraction with CH_2Cl_2(1:1) (GG) Extraction with EtOAc(1:1) (HH) Extraction with BuOH(1:1) (II) Hexane fraction 12.3128(g) (JJ) CH_2CI_2 fraction 8.2564(g) (KK) EtOAc fraction 2.6301(g) (LL) BuOH fraction 30(g) (MM) H_2O fraction 100(g)본 발명은 개암나무 화분 추출물을 유효성분으로 함유하는 항염증, 항산화 및 항균 효과를 지닌 건강 기능성 조성물에 관한 것이다. 더욱 상세하게는 개암나무의 화분을 70% 에탄올로 추출한 후 이에 헥산, 디클로로메탄, 에틸아세테이트, 부탄올을 첨가 혼합시켜 수득된 추출 분획 중 디클로로메탄 분획 및 에틸아세테이트 분획을 유효성분으로 함유하는 항염증, 항산화 및 항균 효과를 지닌 개암나무 화분 추출물 유래 건강 기능성 조성물에 관한 것이다.
