Hany Mohamed Yehia,Reem Atta Alajmi,Hatem Salama Mohamed Ali,Manal Fawzy Elkhadragy,Dina Mahmoud Metwally Hasanin,Mohamed Fekry Serag El-Din,Manal Ahmed Gasmelseed Awad
申请号：
US16137466
公开号：
US10542758B1
申请日:
2018.09.20
申请国别(地区):
US
年份:
2020
代理人:
摘要：
The methanol extract of grape seed nanoparticles is prepared from grape seeds washed in distilled water and oven-dried at 60° C. for 12 hours. The seeds are milled or ground to a powder and sieved to a maximum size of 0.355 mm. The powder is added to concentrated HCl and stirred at 3000 rpm at 30° C. for one hour, and then distilled water is added with stirring for an additional 2 hours. The mixture is filtered, and the marc is dried to recover grape seed nanoparticles. The nanoparticles are added to methanol at the rate of 100 mg/ml, left in a shaker for 24 hours at room temperature, centrifuged, filtered, and the resulting extract (the supernatant) is recovered. Agar well diffusion testing showed that the nanoparticle extract exhibited greater antibacterial activity than a methanol extract of grape seeds alone, and testing showed greater antioxidant levels in the nanoparticle extract as well.
