The present invention relates to a method of producing an extract of Hippophae rhamnoides L. fruit by using ultrasonic waves, in which a mixture containing Hippophae rhamnoides L. fruit and a solvent is subjected to an ultrasonic extraction to extract a natural material from the Hippophae rhamnoides L. fruit and a natural extract of a Hippophae rhamnoides L. fruit produced by the method. The method of the present invention not only improves extraction yield compared to the conventional extraction process, but also maximizes the contents of effective bioactive components in Hippophae rhamnoides L. fruits, including polyphenols and flavonoids, and accordingly, the extract produced by the method of the present invention may be beneficially used in food, drug, and cosmetic industries.COPYRIGHT KIPO 2017본 발명은 갈매보리수 열매에 용매를 혼합한 혼합물을 초음파 추출하여 상기 갈매보리수 열매로부터 천연물을 추출하는 초음파를 이용한 갈매보리수 열매 추출물의 제조방법을 제공한다.