A kind of culture medium and culture method for Antrodia cinnamomea culture is provided. Said culture medium contains cereal composition as a matrix, and nutrient solution containing carbon source, nitrogen source, and peptide and flavonoids as a physiological regulator. Said culture method is to graft Antrodia cinnamomea to the above-described sterilized culture medium and culture for a period of time at room temperature so that Antrodia cinnamomea will generate mycelium, fruiting body and fruiting body-like structure. According to said culture medium and culture method, it is possible, with respect to the natural environment, within a short time to culture the high physiological active ingredient content and a wide diversity of Antrodia cinnamomea. In addition, the ingredients of the culture medium are common and easily accessible, so no need to worry about the shortage of raw materials. The environmental parameters including temperature and humidity in the culture process do not need any special regulation, which is not only easy to promote for application, but can save personnel and costs during the culture process.一種用於培養牛樟芝之培養基與培養方法,該培養基是包含作為基質的穀類組合物,及含有碳源、氮源、作為生理調節物質的胜肽與類黃酮化合物的營養液;該培養方法是將牛樟芝接種至滅菌後的上述培養基,並在室溫下培養一段時間使牛樟芝生成菌絲體、子實體及半子實體。藉由該培養基與該培養方法,能夠在相對於自然環境中短的時間內培養出生理活性成份含量高且種類多元的牛樟芝,且培養基的成份普遍、容易取得,故不需擔心原料匱乏的問題;培養過程中的溫濕度等環境參數不需特別調控,不僅易於推廣應用,更能夠節省培養過程中的人事與成本。
