Disclosed herein are nucleic acid molecules comprising one or more nucleic acid sequences that encode a mutated consensus LEMDl antigen. Vectors, compositions, and vaccines comprising one or more nucleic acid sequences that encode a mutated consensus LEMDl antigen are disclosed. Methods of treating a subject with a LEMDl -expressing tumor and methods of preventing a LEMDl -expressing tumor are disclosed. Mutated consensus LEMDl antigen is disclosed.