by easy to see the position of the surface of the target organ such as the extraction target, the target organ surrounds me and also to prevent damage to the nerve is in. An irradiation unit 3, 7 for irradiating illumination light to body tissue of the reflected light reflected by the irradiated illumination light living tissue by the irradiation unit 3, 7, the absorption characteristic of the -carotene than the absorption characteristics of hemoglobin providing an imaging unit 8 for acquiring a reflected light image by photographing a reflected light of higher wavelength bands also, the biological observation apparatus 1 and a display section 6 for displaying the reflected light image acquired by the imaging unit 8.摘出対象等の対象臓器の表面の位置を見やすくして、対象臓器を取り囲んでいる神経の損傷を未然に防止する。生体組織に照明光を照射する照射部3,7と、該照射部3,7により照射された照明光が生体組織により反射された反射光の内、βカロテンの吸収特性がヘモグロビンの吸収特性よりも高い波長帯域の反射光を撮影して反射光画像を取得する撮像部8と、該撮像部8により取得された反射光画像を表示する表示部6とを備える生体観察装置1を提供する。