The present invention is a method for producing osteoblasts from human iPS cells(1) process of inducing the formation of embryoid bodies by non adhering culture of undifferentiated human iPS cells(2) process of inducing differentiation of human iPS cells into mesodermal cells by non adhering culture of embryoid bodies of human iPS cells obtained in the above process(3) process of inducing differentiation of mesenchymal cells of human iPS cells in vitro (2) by non adhering culture to induce osteoblast differentiationTo provide a method.本発明はヒトｉＰＳ細胞から骨芽細胞塊を製造する方法であって、（１）未分化ヒトｉＰＳ細胞を非接着培養して、胚様体の形成を誘導する工程、（２）上記工程（１）で得られたヒトｉＰＳ細胞の胚様体を非接着培養して、ヒトｉＰＳ細胞の中胚葉系細胞への分化を誘導する工程、及び（３）上記工程（２）で得られたヒトｉＰＳ細胞の中胚葉系細胞を非接着培養して、骨芽細胞への分化を誘導する工程を含む方法を提供する。
