Disclosed is a method for producing retinal pigment epithelium (RPE) cells. Methods (a): cultivating human pluripotent stem cells in human feeder cell conditioned medium and obtaining a culture population of human pluripotent stem cells (b) cultivating the cultured population of human pluripotent stem cells in a medium containing a differentiation agent and obtaining differentiated cells and (c) The process of culturing the differentiated cells in a medium containing one or more members of the TGF beta superfamily.網膜色素上皮(RPE)細胞を作製する方法を開示する。方法は、(a)ヒトフィーダー細胞馴化培地中でヒト多能性幹細胞を培養し、ヒト多能性幹細胞の培養集団を得る工程;(b)分化作用物質を含む培地中でヒト多能性幹細胞の前記培養集団を培養し、分化細胞を得る工程;および(c)TGFβスーパーファミリーの1つまたは複数のメンバーを含む培地中で前記分化細胞を培養する工程を含む。
