PROBLEM TO BE SOLVED: To provide an improved retina therapy for treatment of degenerative disease of retina.SOLUTION: There is provided a method for isolating retinal pigment epithelium(RPE)-like cell for differentiating human embryonic stem cells and human embryo-derived cells into RPE-like cells and other retinal progenitor cells, comprising (a) a step of culturing hES cells in a medium supporting proliferation of hES cells and trans-differentiation to RPE-like cells (b) a step of selecting cells developing a sign of differentiation along a nerve system from the cells obtained by the step (a) (c) a step of subculturing the cells selected by the step (b) using an enzyme selected from the group consisting of trypsin, collagenase IV, collagenase I and dispase until a pigmented epithelium island is developed or its number is increased and (d) a step of selecting the pigmented or non-pigmented cells subcultured in the step (c) for establishing a high-purity RPE-like culture.COPYRIGHT: (C)2012,JPO&INPIT【課題】網膜変性疾患の治療のため、改良された網膜療法を提供する。【解決手段】ヒト胚性幹細胞およびヒト胚由来細胞を網膜色素上皮(RPE)細胞および他の網膜先祖細胞に分化させるための方法であって、a)hES細胞の増殖およびRPE-様細胞へのトランス分化を支持する培地中でhES細胞を培養し;b)神経系に沿って分化の兆候を呈する工程a)の細胞を選択し;c)色素沈着上皮島が出現し、または数が増加するまで、トリプシン、コラゲナーゼIV、コラゲナーゼI、およびジスパーゼよりなる群から選択される酵素を用いて工程b)で選択された細胞を継代し;次いで、d)高純度RPE-様培養の樹立のために、工程c)で継代した色素沈着または非-色素沈着細胞を選択する;ことを含む、RPE-様細胞を単離する方法。【選択図】なし
