The present invention is related to a Tremella fuciformis extract with anti biotic activity and a manufactruing method thereof. The manufacturing method of the Tremella fuciformis extract comprises the steps of preparing Tremella fuciformis drying the Tremella fuciformis by freezing and grinding the dried Tremella fuciformis to powder adding alcohol into the Tremella fuciformis powder and then sonicating obtaining upper solution from the alcohol comprising the Tremella fuciformis powder after waiting for a while centrifugating theupper solution to obtain supernatant and filtering the supernatant to gain a Tremella fuciformis extract. The Tremella fuciformis extract has functions of anti-Staphylococcus aureus, Propionibacterium acnes and Streptococcus mutans after antibacterial circle experiments.本發明係有關於一種具有抗菌功能之銀耳萃取液及其製備方法,該銀耳萃取液之製備方法,其包含:取提供一銀耳原料;將該銀耳原料冷凍乾燥,並研磨呈粉末狀,以取得一銀耳粉末;將一酒精加入該銀耳粉末中,並以超音波震盪;靜置一預定時間後,取含有該銀耳粉末之該酒精的一上層液;離心該上層液,以取得一上清液;以及過濾該上清液,即得一銀耳萃取液。該銀耳萃取液經抗菌圈測試證實具有抗金黃色葡萄球菌、痤瘡丙酸桿菌以及變形鏈球菌之功效。S11至S16...步驟
