LARGE GENOMIC DNA KNOCK-IN AND USES THEREOF

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专利权人:

THE JACKSON LABORATORY

发明人:

BERGSTROM, DAVID,LEIDY-DAVIS, TIFFANY

申请号：

CA3004497

公开号：

CA3004497A1

申请日:

2016.11.07

申请国别(地区):

CA

年份:

2017

代理人:

摘要：

The present invention provides compositions and methods for utilizing a large capacity cloning vector (e.g., BAC) to carry a large exogenous genomic DNA (about 10-300 kb) flanked by a proximal and distal regions (10 kb) to efficiently insert into the genome of a cell in a CRISPR/Cas9-stimulated homologous recombination. Methods and compositions for microinjecting a large human gene into a mouse zygote to prepare a genetically modified mouse are also provided.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/