Large genomic DNA knock-in and uses thereof

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专利权人:

The Jackson Laboratory

发明人:

Bergstrom, David,Leidy-Davis, Tiffany

申请号：

AU2016349738

公开号：

AU2016349738A1

申请日:

2016.11.07

申请国别(地区):

AU

年份:

2018

代理人:

摘要：

The present invention provides compositions and methods for utilizing a large capacity cloning vector (e.g., BAC) to carry a large exogenous genomic DNA (about 10-300 kb) flanked by a proximal and distal regions (10 kb) to efficiently insert into the genome of a cell in a CRISPR/Cas9-stimulated homologous recombination. Methods and compositions for microinjecting a large human gene into a mouse zygote to prepare a genetically modified mouse are also provided.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/