Robert Jason Christopher Geijskes;Paulo Cezar De Lucca
发明人:
Robert Jason Christopher Geijskes,Paulo Cezar De Lucca
申请号：
US13703846
公开号：
US09447421B2
申请日:
2011.06.22
申请国别(地区):
US
年份:
2016
代理人:
摘要：
The present invention provides methods for Agrobacterium-mediated transformation of sugar cane (Saccharum spp.) comprising introducing a nucleotide sequence of interest into a sugar cane callus tissue or cell thereof via Agrobacterium mediated delivery, wherein the sugar cane callus tissue is less than 28 days post-initiation. The invention further provides methods for transforming a sugar cane callus tissue or cell thereof comprising inoculating the sugar cane callus tissue that is less than 28 days post-initiation with an Agrobacterium comprising a nucleotide sequence of interest to produce an Agrobacterium-inoculated sugar cane tissue or cell thereof； and co-cultivating the Agrobacterium and the sugar cane callus tissue to produce a transformed sugar cane callus tissue or cell thereof.
