QUEENSLAND UNIVERSITY OF TECHNOLOGY;SYNGENTA PARTICIPATIONS AG
发明人:
GEIJSKES, ROBERT JASON CHRISTOPHER,DE LUCCA, PAULO CEZAR
申请号:
EP11798792
公开号:
EP2584886A4
申请日:
2011.06.22
申请国别(地区):
EP
年份:
2013
代理人:
摘要:
The present invention provides methods for Agrobacterium-mediated transformation of sugar cane (Saccharum spp.) comprising introducing a nucleotide sequence of interest into a sugar cane callus tissue or cell thereof via Agrobacterium mediated delivery, wherein the sugar cane callus tissue is less than 28 days post-initiation. The invention further provides methods for transforming a sugar cane callus tissue or cell thereof comprising inoculating the sugar cane callus tissue that is less than 28 days post-initiation with an Agrobacterium comprising a nucleotide sequence of interest to produce an Agrobacterium-inoculated sugar cane tissue or cell thereof; and co-cultivating the Agrobacterium and the sugar cane callus tissue to produce a transformed sugar cane callus tissue or cell thereof.