DE LUCCA PAULO CEZAR,DONG SHUJIE,GEIJSKES ROBERT JASON CHRISTOPHER,DUNDER ERIK MARTIN,SAINZ MANUEL BENITO
申请号：
IN5088/KOLNP/2011
公开号：
IN2011KN05088A
申请日:
2011.12.19
申请国别(地区):
IN
年份:
2016
代理人:
摘要：
The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with an Agrobacterium inoculation suspension, said Agrobacterium comprising a nucleic acid of interest, to obtain an Agrobacterium-inoculated sugar cane tissue or cell thereof b) co-cultivating said Agrobacterium- inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said Agrobacterium-inoculated-inoculated sugar cane tissue or cell thereof and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants.
