Queensland University of Technology;Syngenta Participations AG
发明人:
De Lucca, Paulo Cezar,Dong, Shujie,Geijskes, Robert Jason Christopher,Dunder, Erik Martin,Sainz, Manuel Benito
申请号：
AU2010264445
公开号：
AU2010264445A1
申请日:
2010.06.24
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with an inoculation suspension, said comprising a nucleic acid of interest, to obtain an -inoculated sugar cane tissue or cell thereof； b) co-cultivating said -inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said -inoculated-inoculated sugar cane tissue or cell thereof； and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants.
