PETOLINO JOSEPH F,STRANGE TONYA L,SAMUEL JAYAKUMAR PON,BLUE RYAN C,SIMPSON MATTHEW A
申请号：
IN201617037753
公开号：
IN201617037753A
申请日:
2016.11.04
申请国别(地区):
IN
年份:
2017
代理人:
摘要：
Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.
