Petolino, Joseph F.,Strange, Tonya L.,Samuel, Jayakumar Pon,Blue, Ryan C.,Simpson, Matthew A.
申请号：
AU2015253536
公开号：
AU2015253536A1
申请日:
2015.04.24
申请国别(地区):
AU
年份:
2016
代理人:
摘要：
Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.
