ПЕТОЛИНО Джозеф Ф. (US),СТРЭЙНДЖ Тоня Л. (US),СЭМЬЮЭЛ Джаякумар Пон (US),БЛЮ Райан С. (US),СИМПСОН Мэттью А. (US)
申请号：
RU2016143352
公开号：
RU2016143352A
申请日:
2015.04.24
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.
