PETOLINO, Joseph F.,STRANGE, Tonya L.,SAMUEL, Jayakumar Pon,BLUE, Ryan C.,SIMPSON, Matthew A.
申请号：
EP15785964
公开号：
EP3136842A4
申请日:
2015.04.24
申请国别(地区):
EP
年份:
2017
代理人:
摘要：
Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.
