A gardenia extract, preparation and its use thereof for protecting eye are provided. The preparation includes: crushing a gardenia dry fruits, and then extracting with 40 wt% to 95 wt% ethanol to obtain a first extract； purifying the first extract using a macroporous resin A with 40 wt% to 95 wt% ethanol to obtain the second extract； and the second extract was eluted through a macroporous resin B with 60 wt% to 95 wt% ethanol to obtain a gardenia extract. When the gardenia extract prepared by the method is administered to subjects in need thereof, the visual function of the subjects can be improved.本發明提供一種梔子萃取物、其製備方法及其用於護眼之用途。該製備方法包含以下步驟：將梔子乾果進行粉碎製程之後，以40wt%~95wt%乙醇進行萃取，得到第一萃取物；將第一萃取物利用大孔吸附樹脂A進行吸附，再經40wt%~95wt%乙醇進行沖提，得到第二萃取物；以及將第二萃取物以大孔吸附樹脂B進行吸附，再經60wt%~95wt%乙醇進行沖提，以得到梔子萃取物。當將該方法所製得的梔子萃取物施予給所需個體時，可改善該個體的視覺功能。
