The invention belongs to the field of drug or health care product, and more specifically relates to a gynura formosana Kitam. total flavonoids extract, a preparation method thereof, and applications of the gynura formosana Kitam. total flavonoids extract in treating hyperuricemia. The gynura formosana Kitam. total flavonoids extract comprises, by weight, 80 to 85% of rutin. It is shown by drug effect experiment results that the gynura formosana Kitam. total flavonoids extract is capable of reducing the activity of xanthine oxidase in the livers of hyperuricemia mouse models and inhibiting systhesis of uric acid at a certain degree, and reducing uric acid at a certain degree, and can be taken as a potential drug used for treating hyperuricemia or gout. According to the preparation method, after extraction, a composite enzyme is composed of enzymes of specific composition and specific ratio, and is used for enzymatic hydrolysis； and in addition, macroporous resin extraction condensationand macroporous resin separation purifying are adopted. The HPLC purity of rutin in the gynura formosana Kitam. total flavonoids extract is capable of reaching 80 to 85%.本發明屬於藥品或保健品領域，具體涉及一種白鳳菜總黃酮提取物及其製備方法與治療高尿酸血症的用途。該提取物中，以重量百分含量計，含有80~85%的芸香苷。藥效實驗結果顯示，該提取物可以在一定程度上降低高尿酸血症模型小鼠肝臟內黃嘌呤氧化酶的活性、降低尿酸的合成，具有一定的降尿酸作用，可以作為潛在的治療高尿酸血症或治療痛風的藥物；本發明白鳳菜總黃酮提取物的製備方法，在提取步驟後，通過選擇特定組成、特定配比的酶組成的複合酶進行酶解，進一步通過大孔樹脂萃取濃縮和大孔樹脂分離純化步驟，使得製備得到的白鳳菜總黃酮提取物中芸香苷的HPLC純度可達80~85%。
