The invention belongs to the field of drug or health care product, and more specifically relates to a gynura formosana Kitam. total flavonoids extract, a preparation method thereof, and applications of the gynura formosana Kitam. total flavonoids extract in treating non-alcoholic fatty liver disease. The gynura formosana Kitam. total flavonoids extract comprises, by weight, 80 to 85% of rutin. Itis shown by drug effect experiment results that the gynura formosana Kitam. total flavonoids extract possesses relatively excellent curative effect on non-alcoholic fatty liver disease, is capable ofimproving lipid disorders and fatty degeneration of mice with non-alcoholic fatty liver disease, and can be taken as a potential drug used for treating non-alcoholic fatty liver disease. According tothe preparation method, after extraction, a composite enzyme is composed of enzymes of specific composition and specific ratio, and is used for enzymatic hydrolysis； and in addition, macroporous resinextraction condensation and macroporous resin separation purifying are adopted. The HPLC purity of rutin in the gynura formosana Kitam. total flavonoids extract is capable of reaching 80 to 85%.本發明屬於藥品或保健品領域，具體涉及一種白鳳菜總黃酮提取物及其製備方法與治療非酒精性脂肪肝的用途。該提取物中，以重量百分含量計，含有80~85%的芸香苷。藥效實驗結果顯示，該提取物對非酒精性脂肪肝有較好的治療作用，可顯著改善非酒精性脂肪肝大鼠的血脂紊亂及脂肪變性，因此可以作為潛在的治療非酒精性脂肪肝的藥物；本發明白鳳菜總黃酮提取物的製備方法，在提取步驟後，通過選擇特定組成、特定配比的酶組成的複合酶進行酶解，進一步通過大孔樹脂萃取濃縮和大孔樹脂分離純化步驟，使得製備得到的白鳳菜總黃酮提取物中芸香苷的HPLC純度可達80~85%。
