The invention belongs to the field of drug or health care product, and more specifically relates to a gynura formosana Kitam. total flavonoids extract, a preparation method thereof, and applications of the gynura formosana Kitam. total flavonoids extract in treating alcoholic fatty liver. The gynura formosana Kitam. total flavonoids extract comprises, by weight, 80 to 85% of rutin. Drug effect experiments are carried out taking zebra fish alcoholic fatty liver models as experimental models. It is shown by experiment results that the gynura formosana Kitam. total flavonoids extract possesses protective effect on alcoholic fatty liver, is capable of protecting livers, and can be taken as a potential drug used for treating alcoholic liver injury. According to the preparation method, after extraction, a composite enzyme is composed of enzymes of specific composition and specific ratio, and is used for enzymatic hydrolysis； and in addition, macroporous resin extraction condensation and macroporous resin separation purifying are adopted. The HPLC purity of rutin in the gynura formosana Kitam. total flavonoids extract is capable of reaching 80 to 85%.本發明屬於藥品或保健品領域，具體涉及一種白鳳菜總黃酮提取物及其製備方法與治療酒精性脂肪肝的用途。該提取物中，以重量百分含量計，含有80~85%的芸香苷。本發明進一步以斑馬魚酒精性脂肪肝模型為實驗模型進行藥效實驗研究，實驗結果顯示，本發明白鳳菜總黃酮提取物能夠有效對酒精性肝損傷起到保護效果，具有保肝護肝藥效作用，可以作為潛在的治療酒精性肝損傷的藥物；本發明白鳳菜總黃酮提取物的製備方法，在提取步驟後，通過選擇特定組成、特定配比的酶組成的複合酶進行酶解，進一步通過大孔樹脂萃取濃縮和大孔樹脂分離純化步驟，使得製備得到的白鳳菜總黃酮提取物中芸香苷的HPLC純度可達80~85%。
