FIELD: medicine.SUBSTANCE: invention relates to methods for preparing glycoconjugates suitable for use in medicine. Proposed are methods for the preparation of glycoconjugates having a molecular weight of 1,000 to 20,000 kDa, containing a bacterial capsular polysaccharide conjugated to a carrier protein, wherein said polysaccharide is covalently attached to said carrier protein, comprising the steps of: a) reacting a bacterial capsular polysaccharide, which is derived from S. pneumoniae and selected from capsular polysaccharides of Pn-serotype 3 (pneumococcal serotype 3), Pn-serotype 10A, Pn-serotype 12F and Pn-serotype 33F, with a stable nitroxyl radical compound and 0.1–10 molar equivalents of the oxidizing agent for selective oxidation of the primary hydroxyl groups to obtain an activated saccharide containing aldehyde groups； b) converting said activated bacterial capsular polysaccharide to an amino group of said carrier protein, selected from tetanus, diphtheria, pertussis toxin, Pseudomonas toxin, E. coli, Staphylococcus, Streptococcus or CRM197, at a ratio of saccharide: carrier/protein (wt/wt) from 0.2 to 4 under reductive amination conditions, wherein, after conjugation, the non-reacted aldehyde groups are converted back to primary alcohols and the glycoconjugate is purified. Also, provided is a glycoconjugate obtained by said methods from a capsular polysaccharide of Pn-serotype 12F, compositions comprising said glycoconjugate, and a method for preventing, treating or alleviating bacterial infections caused by serotype 12F S. pneumoniae, a method for inducing a protective immune response against S. pneumoniae infection by administering said composition to a subject.EFFECT: provided are new effective methods for the preparation of glycoconjugates, new glycoconjugates and compositions based on them, effective for the treatment of infections caused by Streptococcus pneumoniae.58 cl, 7 dwg, 5 tbl, 8 exИзобретение относится к способам получения гликоконъюгатов,
